Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2005
Volume 8
Issue 4/volume8
Topic:
Veterinary Medicine
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Niżański W. , Kuropka P. 2005. ULTRASTRUCTURAL CHANGES IN DOG SPERMATOZOA AFTER FREEZING–THAWING OF SEMEN EXTENDED IN TRIS–BASED DILUENT SUPPLEMENTED WITH EQUEX STM, EJPAU 8(4/volume8), #54.
Available Online: http://www.ejpau.media.pl/volume8/issue4/volume8/abs-54.html

ULTRASTRUCTURAL CHANGES IN DOG SPERMATOZOA AFTER FREEZING–THAWING OF SEMEN EXTENDED IN TRIS–BASED DILUENT SUPPLEMENTED WITH EQUEX STM

Wojciech Niżański1, Piotr Kuropka2
1 Department and Clinic of Obstetrics, Ruminant Diseases and Animal Health Care, Wrocław University of Environmental and Life Sciences, Poland
2 Department of Anatomy and Histology, Institute of Histology and Embryology, Agricultural University of Wrocław, Poland

 

ABSTRACT



The aim of the present study was to characterise the ultrastructural changes present in canine spermatozoa after freezing-thawing with the use of extender with addition of surface active agent Equex STM. The experiment was carried out on 10 ejaculates collected from 5 dogs of various breeds. Semen was extended in Tris–based diluent with addition of Equex STM. Glycerolisation started at 5°C. Semen was frozen in 0.5 ml French straws. Results of microscopical examination of frozen-thawed spermatozoa seemed to be excellent. Microscopically assessed post-thaw percentage of progressively motile spermatozoa, percentage of spermatozoa with normal morphology, percentage of cells with intact acrosome and proportion of live spermatozoa after thawing were 60.5 ±4.4, 62.1 ±6.8, 67.7 ±4.8 and 62.6 ±7.7, respectively. Transmission electron microscopic examination of frozen–thawed semen revealed major changes in the morphology of spermatozoa localised predominantly within the acrosome and postacrosomal region of a head. In many cells the acrosome and equatorial segment of a spermatozoal head were damaged. The most common alterations seen in frozen–thawed dog spermatozoa were due to the process of false acrosome reaction. Several degrees of false acrosome reaction were observed: swelling of acrosomes, vesiculation of outer acrosomal membrane and plasma membrane, and complete detachment of the acrosome. The degree and character of deterioration of the acrosome structure of cryopreserved dog spermatozoa extended in Tris–based and Equex STM–supplemented diluent is similar to ultrastructural changes observed in spermatozoa cryopreserved in other Tris extenders without surface–active agent addition.

Key words: semen, spermatozoon, dog, ultrastructure, cryopreservation.

Wojciech Niżański
Department and Clinic of Obstetrics,
Ruminant Diseases and Animal Health Care,
Wrocław University of Environmental and Life Sciences, Poland
pl. Grunwaldzki 49, 50-366 Wrocław, Poland
Phone: (+48 71) 32 05 315
email: nizanski@ozi.ar.wroc.pl

Piotr Kuropka
Department of Anatomy and Histology,
Institute of Histology and Embryology,
Agricultural University of Wrocław, Poland
Kożuchowska 5, 51–631 Wrocław, Poland
Phone: (+48 71) 32 05 796

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.

Main  -  Issues  -  How to Submit  -  From the Publisher  -  Search  -  Subscription