Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2005
Volume 8
Issue 4/volume8
Topic:
Biotechnology
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Żyła K. , Mika M. , Fortuna T. , Szymczyk B. , Czubak M. 2005. EFFECTS OF SESAME SEEDS GERMINATION ON PHYTATE DEGRADATION, IN VITRO NUTRIENT DIGESTIBILITY AND PLASMA LIPID PROFILE IN RATS, EJPAU 8(4/volume8), #53.
Available Online: http://www.ejpau.media.pl/volume8/issue4/volume8/abs-53.html

EFFECTS OF SESAME SEEDS GERMINATION ON PHYTATE DEGRADATION, IN VITRO NUTRIENT DIGESTIBILITY AND PLASMA LIPID PROFILE IN RATS

Krzysztof Żyła1, Magdalena Mika1, Teresa Fortuna2, Beata Szymczyk3, Marta Czubak1
1 Department of Food Biotechnology, University of Agriculture in Cracow, Poland
2 Department of Analysis and Food Quality Evaluation, Agricultural University, Cracow, Poland
3 National Research Institute of Animal Production Department of Animal Nutrition and Feed Science in Aleksandrowice, Poland

 

ABSTRACT



Germination of sesame seeds was used to activate endogenous phytate-degrading enzymes that were expected to dephosphorylate phytate into lower myo-inositol phosphates. Effects of germination were related to the activity of endogenous phytase, chemical composition of seeds and in vitro digestibility of nutrients, as well as phosphorus and calcium metabolic balance and lipid composition in the plasma of rats. The activity of sesame phytase grew from 1.4 FTU/g in raw seeds to 5.0 FTU/g after 36 hours of germination at 30°C and pH 5.5, but only minor changes in the profile of myo-inositol phosphates in germinated seeds were observed. In spite of limited phytate hydrolysis, germination improved in vitro digestibility of phosphorus, calcium, magnesium and carbohydrates, but did not influence digestibility of protein. In rats, germination improved feed intake and feed efficiency (p<0.01), improved HDL/total cholesterol ratio (p<0.05) and lowered concentration of plasma triacyloglicerols p<0.05).

Key words: sesame seed, germination, phytate, phytase, in vitro digestibility, rat, serum lipids.

Krzysztof Żyła
Department of Food Biotechnology,
University of Agriculture in Cracow, Poland
Balicka 122, 30-149 Cracow, Poland
phone/fax: (+4812) 662 4794
email: kzyla@ar.krakow.pl

Magdalena Mika
Department of Food Biotechnology,
University of Agriculture in Cracow, Poland
Balicka 122, 30-149 Cracow, Poland

Teresa Fortuna
Department of Analysis and Food Quality Evaluation,
Agricultural University, Cracow, Poland
Balicka 122, 30-149 Cracow, Poland
email: rrfortun@cyf-kr.edu.pl

Beata Szymczyk
National Research Institute of Animal Production
Department of Animal Nutrition and Feed Science in Aleksandrowice, Poland
32-083 Balice, Poland

Marta Czubak
Department of Food Biotechnology,
University of Agriculture in Cracow, Poland
Balicka 122, 30-149 Cracow, Poland

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.

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