Volume 8
Issue 3/volume8
Biotechnology
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Available Online: http://www.ejpau.media.pl/volume8/issue3/volume8/abs-31.html
DEGRADATION OF PETROLEUM-DERIVED HYDROCARBONS BY ALGINATE-IMMOBILIZED CELLS OF CANDIDA LIPOLYTICA
Elżbieta Bogusławska-W±s1, Katarzyna Czeszejko1, Artur Bartkowiak2, Waldemar D±browski1, Agnieszka Michniewicz1, Katarzyna Szameto1
1 Department of Food Microbiology,
Agricultural University of Szczecin, Poland
2 Department of Packaging and Polymers,
Agricultural University of Szczecin, Poland
ABSTRACT
The objective of the studies was to evaluate possibilities of petroleum oil biodegradation by free and immobilized cells of C. lipolytica. Immobilization was conducted in 2% sodium alginate coated/non-coated with 1.0% chitosane. The elimination process involved 94.1% of the total amount of introduced hydrocarbons in cultures containing free cells of C. lipolytica and 96.5% and 96.1% of hydrocarbons in cultures containing immobilized cells. Statistically significant differences were observed for the rate of hydrocarbon degradation. The remains of traced substratum were at the level of 5.1% and 5.4% in cultures with encapsulated yeasts whereas comparable amounts of petroleum hydrocarbons (4.9%) were detected on 21st day of the experiment.
Key words: cell immobilization, C. lipolytica, biodegradation of hydrocarbons.
Elżbieta Bogusławska-W±s
Department of Food Microbiology,
Agricultural University of Szczecin, Poland
Papieża Pawa VI 3, Szczecin, Poland
email: ewas@tz.ar.szcecin.pl
Katarzyna Czeszejko
Department of Food Microbiology,
Agricultural University of Szczecin, Poland
Papieża Pawa VI 3, Szczecin, Poland
Artur Bartkowiak
Department of Packaging and Polymers,
Agricultural University of Szczecin, Poland
Waldemar D±browski
Department of Food Microbiology,
Agricultural University of Szczecin, Poland
Papieża Pawa VI 3, Szczecin, Poland
Agnieszka Michniewicz
Department of Food Microbiology,
Agricultural University of Szczecin, Poland
Papieża Pawa VI 3, Szczecin, Poland
Katarzyna Szameto
Department of Food Microbiology,
Agricultural University of Szczecin, Poland
Papieża Pawa VI 3, Szczecin, Poland
Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.