Volume 22
Issue 2/volume22
Agronomy
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
DOI:10.30825/5.ejpau.175.2019.22.2 , EJPAU 22(2/volume22), #05.
Available Online: http://www.ejpau.media.pl/volume22/issue2/volume22/art-05.html
EFFECT OF EXTRACTION METHOD AND DNA QUALITY ON THE RELIABILITY OF MOLECULAR DETECTION OF BRADYRHIZOBIUM JAPONICUM IN SOYBEAN RHIZOSPHERE
DOI:10.30825/5.EJPAU.175.2019.22.2
Anna Baturo-Cieśniewska1, Giuseppe Loddi2, Janusz Prusiński3, Aleksander Łukanowski1
1 Laboratory of Phytopathology and Molecular Mycology, Department of Biology and Plant Pathology, Faculty of Agriculture and Biotechnology, UTP University of Science and Technology, Bydgoszcz, Poland
2 Consortium UNO, Faculty of Biology and Pharmacy, University of Cagliari, Oristano, Italy
3 Department of Agrotechnology, Faculty of Agriculture and Biotechnology, UTP University of Science and Technology, Bydgoszcz, Poland
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Key words:
Bradyrhizobium japonicum, DNA extraction, PCR inhibitors, real-time PCR, soil.
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Anna Baturo-Cieśniewska
Laboratory of Phytopathology and Molecular Mycology, Department of Biology and Plant Pathology, Faculty of Agriculture and Biotechnology, UTP University of Science and Technology, Bydgoszcz, Poland
Kordeckiego St. 20
85-225 Bydgoszcz
Poland
email: baturo-a@utp.edu.pl
Giuseppe Loddi
Consortium UNO, Faculty of Biology and Pharmacy, University of Cagliari, Oristano, Italy
Carmine St.
09170 Oristano
Italy
Janusz Prusiński
Department of Agrotechnology, Faculty of Agriculture and Biotechnology, UTP University of Science and Technology, Bydgoszcz, Poland
Kordeckiego St. 20
85-225 Bydgoszcz
Poland
email: janusz.prusinski@utp.edu.pl
Aleksander Łukanowski
Laboratory of Phytopathology and Molecular Mycology, Department of Biology and Plant Pathology, Faculty of Agriculture and Biotechnology, UTP University of Science and Technology, Bydgoszcz, Poland
Kordeckiego St. 20
85-225 Bydgoszcz
Poland
Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.