Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2018
Volume 21
Issue 4/volume21
Topic:
Agronomy
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Tomaszewska-Sowa M. , Siwik-Ziomek A. , Figas A. , Bocian K. 2018. ASSESSMENT OF METAL NANOPARTICLE-INDUCED MORPHOLOGICAL AND PHYSIOLOGICAL CHANGES IN IN VITRO CULTURES OF RAPESEED (BRASSICA NAPUS L.)
DOI:10.30825/5.ejpau.166.2018.21.4, EJPAU 21(4/volume21), #07.
Available Online: http://www.ejpau.media.pl/volume21/issue4/volume21/abs-07.html

ASSESSMENT OF METAL NANOPARTICLE-INDUCED MORPHOLOGICAL AND PHYSIOLOGICAL CHANGES IN IN VITRO CULTURES OF RAPESEED (BRASSICA NAPUS L.)
DOI:10.30825/5.EJPAU.166.2018.21.4

Magdalena Tomaszewska-Sowa1, Anetta Siwik-Ziomek2, Anna Figas1, Karol Bocian1
1 Department of Agricultural Biotechnology, University of Science and Technology, Bydgoszcz, Poland
2 Department of Biogeochemistry and Soil Science, University of Science and Technology, Bydgoszcz, Poland

 

ABSTRACT

To understand the mechanisms involved in plant responses to metal nanoparticles, the morphological and physiological changes in rapeseed plants (Brassica napus L.) were determined. The sterile seeds were inoculated onto the MS medium (sterilized and unsterilized) containing silver, copper and gold nanoparticles at the concentration of 20 ppm. Silver nanoparticled did not inhibit the germination process as opposed to cooper nanoparticles. The nanoparticles did not affect the roots length. The seedlings grown on the medium supplemented with nanoparticles were significantly shorter in comparison to the control. Nanosilver without autoclave sterilization reduced the plant height. The total chlorophyll, the ratio of chl a / chl b and carotenoid content decreased after the exposure to metal nanoparticles. Additionally, it was found that the nanoparticles of gold increased the activity of catalase, as compared with the control. There is a growing number of research of the biological effects of nanoparticles on higher plants. The application of nanoparticles in in vitro cultures can be very important to facilitate understanding the nanoparticles toxicity for plants.

Key words: catalase, chlorophyll, nanocopper, nanogold, nanosilver, oxidative stress.

Magdalena Tomaszewska-Sowa
Department of Agricultural Biotechnology, University of Science and Technology, Bydgoszcz, Poland

email: magda@utp.edu.pl

Anetta Siwik-Ziomek
Department of Biogeochemistry and Soil Science, University of Science and Technology, Bydgoszcz, Poland

email: ziomek@utp.edu.pl

Anna Figas
Department of Agricultural Biotechnology, University of Science and Technology, Bydgoszcz, Poland


Karol Bocian
Department of Agricultural Biotechnology, University of Science and Technology, Bydgoszcz, Poland

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.

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