Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2018
Volume 21
Issue 4/volume21
Topic:
Fisheries
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Panicz R. , Sadowski J. , Hofsoe-Oppermann P. , Półgęsek M. 2018. A NEW IDENTIFICATION METHOD FOR CLARIAS GARIEPINUS, CLARIAS MACROCEPHALUS AND THEIR HYBRIDS
USING PCR-RFLP
DOI:10.30825/5.ejpau.164.2018.21.4, EJPAU 21(4/volume21), #05.
Available Online: http://www.ejpau.media.pl/volume21/issue4/volume21/abs-05.html

A NEW IDENTIFICATION METHOD FOR CLARIAS GARIEPINUS, CLARIAS MACROCEPHALUS AND THEIR HYBRIDS
USING PCR-RFLP
DOI:10.30825/5.EJPAU.164.2018.21.4

Remigiusz Panicz1, Jacek Sadowski2, Paulina Hofsoe-Oppermann2, Mirosław Półgęsek2
1 Department of Meat Technology, Faculty of Food Technology and Fisheries,
West Pomeranian University of Technology, Szczecin, Poland
2 Division of Aquaculture, Faculty of Food Technology and Fisheries, West Pomeranian University of Technology, Szczecin, Poland

 

ABSTRACT

A high tolerance to a wide spectrum of environmental factors and excellent taste of its meat explain why the African sharptooth catfish (Clarias gariepinus) is widely used for creating interspecific hybrids with the broadhead catfish (Clarias macrocephalus), a species which is characterized by a high growth rate. However, when the spawning stock on a fish farm is being supplemented with new individuals, there is a serious concern about introducing hybrids instead of purebred individuals. Therefore, it is necessary to develop a quick and reliable method of hybrid identification. In the presented study species diversity was assessed based on PCR and sequence analysis of the internal transcribed spacer 1 (ITS1) and cytochrome oxidase subunit 1 (COI) sequences. Additionally, three restriction enzymes (Fok I, Eco47I and HhaI) were selected and analysed with PCR-RFLP of COI sequences. ITS1 sequence comparisons revealed 0.64% diversity between analysed species and were submitted into GenBank database (KJ136021 and KJ136022). Analysis of COI sequences revealed over 14% variation between two species and based on obtained species-characteristic restriction patterns, a PCR-RFLP test has been developed.

Key words: catfish hybrids, Claridae, cytochrome oxidase subunit I, internal transcribed spacer 1, species delineation.

Remigiusz Panicz
Department of Meat Technology, Faculty of Food Technology and Fisheries,
West Pomeranian University of Technology, Szczecin, Poland
Kazimierza Królewicza 4
71-550 Szczecin
Poland

email: rpanicz@zut.edu.pl

Jacek Sadowski
Division of Aquaculture, Faculty of Food Technology and Fisheries, West Pomeranian University of Technology, Szczecin, Poland
Kazimierza Królewicza 4
71-550 Szczecin
Poland

email: jsadowski@zut.edu.pl

Paulina Hofsoe-Oppermann
Division of Aquaculture, Faculty of Food Technology and Fisheries, West Pomeranian University of Technology, Szczecin, Poland
Kazimierza Królewicza 4
71-550 Szczecin
Poland

email: phofsoe@zut.edu.pl

Mirosław Półgęsek
Division of Aquaculture, Faculty of Food Technology and Fisheries, West Pomeranian University of Technology, Szczecin, Poland
Kazimierza Królewicza 4
71-550 Szczecin
Poland
email: mpolgesek@zut.edu.pl

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.

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