EJPAU 2018. Mazurek M. , Siekierzyńska A. EFFICIENT IN VITRO PROPAGATION OF VACCINIUM VITIS-IDAEA L. PLANTS ON THE DOUBLE PHASE MEDIUM DOI:10.30825/5.ejpau.160.2018.21.4

Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

2018
Volume 21
Issue 4/volume21

Topic:

Horticulture

ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES

Mazurek M. , Siekierzyńska A. 2018. EFFICIENT IN VITRO PROPAGATION OF VACCINIUM VITIS-IDAEA L. PLANTS ON THE DOUBLE PHASE MEDIUM
DOI:10.30825/5.ejpau.160.2018.21.4, EJPAU 21(4/volume21), #01.
Available Online: http://www.ejpau.media.pl/volume21/issue4/volume21/abs-01.html

EFFICIENT IN VITRO PROPAGATION OF VACCINIUM VITIS-IDAEA L. PLANTS ON THE DOUBLE PHASE MEDIUM
DOI:10.30825/5.EJPAU.160.2018.21.4

Marzena Mazurek, Aleksandra Siekierzyńska
Department of Plant Biotechnology and Physiology, Faculty of Biology and Agriculture, University of Rzeszow, Poland

ABSTRACT

The aim of the presented study was to investigate the impact of double phase medium in comparison with single phase medium on the growth and development of axillary and adventitious shoots in the lingonberry in vitro culture. Two-node explants were prepared from shoots collected from mother in vitro cultures and were placed on single phase and double phase Zimmerman and Boome medium. For double phase medium, 5 ml of liquid medium was added. The composition of the liquid medium was the same as solid one minus agar. The liquid medium was added after positioning the explants horizontally.
Shoots of axillary origin were used as explants. At the end of each of 3 passages, the number of axillary and adventitious shoots was determined. We demonstrated in presented research that the double phase medium significantly stimulated multiplication and elongation of axillary and adventitious shoots in comparison with single phase medium. Mean number of shoots was about twice higher for double phase medium. Application of double phase medium is a simple method which does not require special equipment. With a minimal increase of costs, it significantly increases the productivity of cultures. Therefore, it may be used successfully in micropropagation of lingonberry plants.

Key words: adventitious shoots, axillary shoots, lingonberry culture, micropropagation.

Marzena Mazurek
Department of Plant Biotechnology and Physiology, Faculty of Biology and Agriculture, University of Rzeszow, Poland
Ćwiklińskiej Str. 2
35-601 Rzeszów
Poland
email: marzena.guty@poczta.onet.pl

Aleksandra Siekierzyńska
Department of Plant Biotechnology and Physiology, Faculty of Biology and Agriculture, University of Rzeszow, Poland
Ćwiklińskiej Str. 2
35-601 Rzeszów
Poland

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