EJPAU 2012. Kaliński K. , Marycz K. , Czogała J. , Serwa E. MAST CELL IDENTIFICATION AND VISUALIZATION BY MEANS OF ELECTRON MICROSCOPY, IMMUNOHISTOCHEMISTRY AND SPECIAL STAINING METHODS IN THE COURSE OF CANINE ATOPIC DERMATITIS

Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

2012
Volume 15
Issue 1/volume15

Topic:

Veterinary Medicine

ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES

Kaliński K. , Marycz K. , Czogała J. , Serwa E. 2012. MAST CELL IDENTIFICATION AND VISUALIZATION BY MEANS OF ELECTRON MICROSCOPY, IMMUNOHISTOCHEMISTRY AND SPECIAL STAINING METHODS IN THE COURSE OF CANINE ATOPIC DERMATITIS, EJPAU 15(1/volume15), #03.
Available Online: http://www.ejpau.media.pl/volume15/issue1/volume15/abs-03.html

MAST CELL IDENTIFICATION AND VISUALIZATION BY MEANS OF ELECTRON MICROSCOPY, IMMUNOHISTOCHEMISTRY AND SPECIAL STAINING METHODS IN THE COURSE OF CANINE ATOPIC DERMATITIS

Krzysztof Kaliński¹, Krzysztof Marycz², Joanna Czogała¹, Ewa Serwa¹
¹ Electron Microscope Laboratory, University of Environmental and Life Sciences in Wrocław, Poland
² Electron Microscope Laboratory, Departament of Animal Hygiene and Ichthyology, Wrocław University of Environmental and Life Sciences, Poland

ABSTRACT

Atopic dermatitis (A D) is a chronic and recurring inflammatory disease with characteristic clinical presentation and significant levels of pruritus. Up to 10% of the canine population is affected atopic dermatitis. Despite many years of scientific research, there are still many of aspects of atopic pathogenesis which require further explanation. The aim of the study was the attempt to present the possibility of displaying mast cell populations in canine atopic dermatitis by using different staining protocols and microscopy techniques. The study was conducted on biopsy material collected from two groups of dogs: atopic and healthy controls. The material was tested by means of light microscopy. Additional study was conducted using TEM - Transmission Electron Microscope. A range of staining methods, as well as possible use of different microscopes types in order to identify and evaluate mast cell population in canine atopic skin, are presented and compared. Depending on the technique used it is possible to evaluate numbers, topography and activity of mast cells in canine skin.

Key words: mast cells, canine atopic dermatitis, dogs, TEM, AD.

Krzysztof Kaliński
Electron Microscope Laboratory,
University of Environmental and Life Sciences in Wrocław, Poland
Kożuchowska 5b, 51-631 Wrocław, Poland

Krzysztof Marycz
Electron Microscope Laboratory,
Departament of Animal Hygiene and Ichthyology,
Wrocław University of Environmental and Life Sciences, Poland
Kożuchowska 5b, 50-375 Wrocław, Poland
Phone: +48 71 320 58 88
email: krzysztofmarycz@interia.pl

Joanna Czogała
Electron Microscope Laboratory,
University of Environmental and Life Sciences in Wrocław, Poland
Kożuchowska 5b, 51-631 Wrocław, Poland

Ewa Serwa
Electron Microscope Laboratory,
University of Environmental and Life Sciences in Wrocław, Poland
Kożuchowska 5b, 51-631 Wrocław, Poland

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.