Volume 13
Issue 4/volume13
Biotechnology
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Available Online: http://www.ejpau.media.pl/volume13/issue4/volume13/abs-11.html
STREPTOMYCES NIGELLUS AS A BIOCONTROL AGENT OF TOMATO DAMPING-OFF DISEASE CAUSED BY PYTHIUM ULTIMUM
Selim Mohsen Helmy1, Kameil Zeinat2, Saad Abd-elnaby Mahmoud 1, Saad Moataza1, Morsi Nagwa2, Hasabo Amany1
1 Microbial Chemistry Department,
National Research Center, Cairo, Egypt
2 Faculty of Science, Cairo University, Cairo, Egypt
ABSTRACT
A collection of about 15 strains of Streptomyces belonging to different spp. were screened for their ability to grow on fragmented Pythium ultimum mycelia and to produce metabolites that inhibit the growth of this plant pathogenic fungi. Some factors affecting level of growth and fungal cell wall lytic enzymes formation by the most active Streptomyces strains were carried out. On the other hand, Streptomyces nigellus NRC10 showed a strong in-vitro antagonism against P. ultimum in plate assay by producing antifungal metabolites. Furthermore, plant growth chamber study
was also carried out to test suppression of damping off disease by the selected strain. The results showed that percentage of diseased tomato plant was greatly reduced in P.ultimum infested soil when treated with this strain.
Key words: biocontrol, damping-off, Pythium ultimum, Streptomyces nigellus.
Selim Mohsen Helmy
Microbial Chemistry Department,
National Research Center, Cairo, Egypt
Kameil Zeinat
Faculty of Science, Cairo University, Cairo, Egypt
Saad Abd-elnaby Mahmoud
Microbial Chemistry Department,
National Research Center, Cairo, Egypt
Saad Moataza
Microbial Chemistry Department,
National Research Center, Cairo, Egypt
email: Moataza_saad@yahoo.com
Morsi Nagwa
Faculty of Science, Cairo University, Cairo, Egypt
Hasabo Amany
Microbial Chemistry Department,
National Research Center, Cairo, Egypt
Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.