Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

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Wessely-Szponder J. , Kloc K. 2010. THE INFLUENCE OF ISOPRIVET ON NEUTROPHIL SECRETORY ACTION IN THE COURSE OF BRD IN CALVES, EJPAU 13(4/volume13), #05.
Available Online: http://www.ejpau.media.pl/volume13/issue4/volume13/abs-05.html

ABSTRACT

Bovine respiratory disease (BRD) is a multifactorial disease, which causes major economical loses in the cattle farms. Neutrophils play a role in the host defence but during prolonged or excessive inflammation they are destructive for lung tissues. The excessive degranulation with release of among others elastase, myeloperoxidase, and nitric oxide causes lung injury during BRD in calves. The purpose of the study was to investigate the influence of different doses (from 50 to 1000 µg/ml) of isoprinosine (Isoprivet) on the secretory action of neutrophils isolated from calves suffering from BRD. Neutrophil degranulation was assessed by elastase and MPO release, and NO generation.

The elastase release in the group of BRD calves was greater than in healthy calves. The highest level was obtained at the concentration of 1000 µg/ml, and for BRD calves was 98.7±2.39%. MPO release was initially inhibited by low doses of Isoprivet. At the highest concentration of Isoprivet the maximal release was observed in the cultures of neutrophils from both studied groups of calves. Generation of NO by neutrophils from healthy calves increased along with growing concentration of Isoprivet (from the value of 1.28 µM of nitrite in the neutrophil culture without stimulation to 7.2±4.79 µM at 1000 µg/ml). Whereas in the group of BRD calves the inconsiderable decrease of NO production by neutrophils at concentration of 50-500 µg/ml of Isoprivet was observed. The distinct increase of NO generation was revealed only in response to the highest concentration of Isoprivet (12.74±12.53 µM). Thus Isoprivet in low doses had no significant effect on neutrophil secretion or slightly inhibited it. In higher doses this preparation causes excessive release of enzymes and generation of free radicals which may cause lung injury and worsening the course of disease.

Key words: neutrophil, BRD, Isoprivet, elastase.

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Joanna Wessely-Szponder
Department of Pathophysiology,
Faculty of Veterinary Medicine,
Agricultural University in Lublin, Poland
Akademicka 12, 20-033 Lublin, Poland
email: joanna.wessely@up.lublin.pl

Kamila Kloc
Department of Pathophysiology,
Faculty of Veterinary Medicine,
Agricultural University in Lublin, Poland
Akademicka 12, 20-033 Lublin, Poland

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