EJPAU 2007. Nawrot-Chorabik K. INDUCTION AND DEVELOPMENT OF GRAND FIR (<I>ABIES GRANDIS</I> LINDL.) CALLUS IN TISSUE CULTURES

Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

2007
Volume 10
Issue 4/volume10

Topic:

Forestry

ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES

Nawrot-Chorabik K. 2007. INDUCTION AND DEVELOPMENT OF GRAND FIR (ABIES GRANDIS LINDL.) CALLUS IN TISSUE CULTURES, EJPAU 10(4/volume10), #39.
Available Online: http://www.ejpau.media.pl/volume10/issue4/volume10/abs-39.html

INDUCTION AND DEVELOPMENT OF GRAND FIR (ABIES GRANDIS LINDL.) CALLUS IN TISSUE CULTURES

Katarzyna Nawrot-Chorabik
Department of Forest Pathology, Agricultural University of Cracow, Poland

ABSTRACT

The research is a methodological analysis of the first stage of micro-propagation of grand fir (Abies grandis Lindl.), which is included in the group of coniferous tree species introduced in Poland and characterised by a fast growth rate. The experiments were conducted on four types of grand fir explants (mature seeds, mega-gametophytes, mature zygotic embryos, buds collected in springtime from twenty year-old trees from the lower tree crown) with respect to initiation and further development of callus of this species. Nine experiments were conducted, where the following issues were analysed: efficiency of the disinfecting agent and the time of its operation on the percentage of grand fir explants of various origins not infected by bacteria or fungi, impact of media and growth regulators contained in the media on the initiation and development of callus and initiation of adventitious buds. Moreover, the research included the impact of the fungistatic and bacteriostatic agent – PPM and the conditions of conducting breeding (in light and in the dark) on callogenesis and organogenesis. The results of the research have shown that the best explants for in-vitro breeding of grand fir are zygotic embryos isolated from mature seeds (average frequency of regeneration 44.5%) and mega-gametophytes (average frequency of regeneration 10.3%).

Key words: Abies grandis; Embryonal suspensor mass (ESM); Plant Preservative Mixture (PPM); Somatic embryogenesis, Tissue culture.

Katarzyna Nawrot-Chorabik
Department of Forest Pathology,
Agricultural University of Cracow, Poland
Al. 29-Listopada 46, 31-425 Cracow, Poland
email: rlnawrot@cyf-kr.edu.pl

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