Volume 1
Issue 1
Environmental Development
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Available Online: http://www.ejpau.media.pl/volume1/issue1/environment/art-01.html
EFFICIENCY OF WASTEWATER TREATMENT FOR MICROBIOLOGICAL CONTAMINANTS IN THE INITIAL STAGE OF A PLANT-SOIL TREATMENT PLANT´S OPERATION.
Krzysztof Kuczewski, Joanna Kercel-Cwalińska, Edmund Solecki, Iwona Nowak
The article presents research results gained when purifying living economic sewage in plant soil water treatment of 24 hour flow capacity of 110 m3.
Key words:
sewage, plant – soil water – treatment plant, treatment effects.
INTRODUCTION
Some environmental engineers share an unfounded opinion that the efficiency of plant-soil treatment plants in purifying wastewater does not comply with the current regulations, especially in the winter half-year[12].There is a decree (annex 2) that specifies the highest permissible indexes of contamination in sewage released into water and soil. It does not specify, however, permissible levels of bacteriological contaminants. Annex 1 of the decree, referring to the contamination of surface waters divided into three classes of purity, at item 57 mentions faecal coliforms and values are given with reference to the class of water purity.Table 1 presents some chosen values of contamination indexes for surface waters and the highest permissible values in sewage released into water and soil. Data included in Table 1 will be useful for interpreting research results.
Table 1. Chosen contamination indexes for surface waters and highest permissible values for sewage released into water and soil |
Index |
Unit |
Class of water purity |
Sewage |
||
I |
II |
III |
|||
BOD5 |
mg O2/dm3 |
≤ 4 |
≤ 8 |
≤ 12 |
30.0 |
CODCr |
mg O2/ dm3 |
≤ 25 |
≤ 70 |
≤ 100 |
150.0 |
Ammonium nitrogen |
mg NNH4/ dm3 |
≤ 1 |
≤ 3 |
≤ 6 |
6.0 |
Kjeldahl nitrogen |
mg N/ dm3 |
≤ 5 |
≤ 10 |
≤ 15 |
30.0 |
Total phosphorus |
mg P/ dm3 |
≤ 0.1 |
≤ 0.25 |
≤ 0.4 |
5.0 |
Total suspended solids |
mg/ dm3 |
≤ 20 |
≤ 30 |
≤ 50 |
50.0 |
Faecal coli titre |
- |
≥ 1 |
≥ 0.1 |
≥ 0.01 |
- |
The newly-constructed plant-soil treatment plant in the village of Brzezno, designed by K. Kuczewski and J. Paluch, is the first of its kind ever built in Poland. Its main function is the treatment of sewage rather than its agricultural application. The designers went into a lot of trouble to obtain the consent for construction and for releasing treated sewage. Those difficulties resulted from the lack of recorded research results connected with the efficiency of sewage treatment in similar conditions in Poland. As there are no formal requirements regarding the bacteriological quality of treated sewage there are no systematical studies of the efficiency of eliminating bacteriological contaminants in the treatment process. According to Imhoff [8] the highest index of bacteria removal in the treatment process can be achieved by the activated sludge method or by treatnig sewage in soil plants (Table 2).
Table 2. Efficiency ocf different ways of sewage treatment (according to Imhoff) [8] |
Way of treatment |
BOD5 |
Reduction of suspended solids, % |
Bacteria
|
1.Fine sieves |
5-10 |
2-20 |
10-20 |
2. Chlorination of raw sewage or of sewage from primary settling tanks |
15-30 |
- |
90-95 |
3. Primary settling tanks |
25-40 |
40-70 |
25-75 |
4. Floculation tanks |
40-50 |
50-70 |
- |
5. Coagulation |
50-85 |
70-90 |
40-80 |
6. Highly-loaded deposits |
65-90 |
65-92 |
70-90 |
7. Low-loaded deposits |
80-95 |
70-92 |
90-95 |
8. Highly loaded activated sludge |
50-75 |
80 |
70-90 |
9. Low-loaded activated sludge |
75-95 |
85-95 |
90-98 |
10. Soil filters |
90-95 |
85-95 |
95-98 |
11. Chlorination of biologically treated sewage |
- |
- |
98-99 |
Those two methods make it possible to obtain a very considerable decrease in BOD5. Further lowering of bacteria levels in treated sewage can be achieved through disinfection, e.g. chlorination or exposure to UV. One should realise, however, that indexes of contamination lower than those mentioned in the Decree [12]do not mean that the sewage is safe for people.
For sanitary and hygienic control of water and soil indicator bacteria are used, e.g. E. Coli which, as saprophytes, live in human and animal digestive systems. Their presence in water or sewage suggests there is a possibility of contamination with faecal matter.
Considerable numbers of E. Coli in water (sewage) also suggest a high probability of contamination with pathogenic microorganisms.
The purpose of research was to assess the efficiency of sewage treatment in a plant-soil treatment plant in reference to bacteriological contaminants.
The most probable number of E. Coli and coli titre in raw and treated sewage from different parts of the plant were used as indicators. For assessing general efficiency of sewage treatment in this type of treatment plant such indexes of chemical contamination as CODCr, BOD5, Kjeldahl nitrogen, ammonia nitrogen and total suspended solids were also considered.
The studied treatment plant was in the initial stage of operation. The research period (November, December 1997) is considered to be the time when microorganisms participating in the decomposition of sewage are less active, which results in lower efficiency of the treatment.
PLACE OF RESEARCH
When field tests were carried out the plant-soil treatment plant in Brzeźno near Prusice was in its initial stage of operation. It processes sewage from the sewered part of Brzeźno collected in two septic tanks. A flow chart of the sewage system and the treatment plant in Brzeźno are shown in Fig.1. Sewage from one part of the village is gravitationally collected in a septic tank (P-1) and pumped from the other part of the village (from tank P-1) to tank P-2. All sewage is pumped from tank P-2 to the accumulation-dispenser tank which has a capacity of 110 m3, equal to the volume of sewage produced in a village settlement in 24 hrs. After the accumulation-dispenser tank is filled by means of a syphon system, which takes about one hour, the sewage automatically flows into a distributor and then into one of 21 units for collecting sewage. The surface of wetlands was drained, sown with a mixture of grass and planted with poplar cuttings. When sewage has permeate d through the biologically active layer of the soil it is collected by a drainage system and directed to a nearby ditch. The drainage system is situated 1metre below the surface. The plant's subsurface base is built of quarternary deposits consisting of yellowish-gray medium and coarse sands constituting a continuous layer that changes in depth from 1.0 to 1.5 m. Below, there is cohesive soil, i.e. clays, fine clays and sand clays.
Fig. 1. Diagram of Brzeźno plant-soil treatment plant in Brzeźno near Prusice |
![]() |
The treatment plant's area is adjusted to the planned diurnal volume of sewage, i.e.110 m3 and equals 2.30 ha. The wetlands were so designed that the diurnal volume of sewage is collected by a single unit where it stays for no longer than 24 hrs. Theoretically the rotation of inflows occurs every three weeks, so one unit is fed with sewage every 21 days. Should such needs arise, it is possible to decrease the inlowing dose by shortening the rotation time to 7 - 10 days and simultaneous feeding of two or three units. The wetlands area is divided into four segments and sewage wells have been installed (Fig.1), which makes it possible to assess treatment efficiency in each individual segment. The area of each unit equals 1150 m2 and maximum concentration of treated sewage flowing out of the unit happens ca 2.5 hrs after the inflow.
Maximum yearly design dose of sewage equals 1730 mm, i.e.17300 m3/ha. In Nov. 1996, due to lower than expected amount of sewage, a decision was made to use the first segment of the plant, as far as Well 5 (Fig.1). In the winter half-year that part of the plant was loaded with sewage according to the technical design. Any surplus of inflowing sewage was directed to the other part. Total sewage fed into particular segments until the date of research is shown in Table 3.
Table 3. Total volume of sewage in m3 taken by particular segments of the treatment plant until the day of research |
No |
Segment |
Date of research |
|
09.11.1997 |
02.12.1997 |
||
1 2 3 4 |
1 2 3 4 |
8360 5060 4070 3795 |
8690 5280 4290 4125 |
5 |
∑ 1+2+3+4 |
21285 |
22385 |
METHOD AND RESULTS
Field tests of the efficiency of sewage treatment were carried out in a plant-soil treatment plant in its normal operation. Samples for chemical and bacteriological analyses were taken at the following points:
- Well 5 - treated sewage coming from the first segment of the plant,
- Well 4 - a mixture of treated sewage from segment 1 and 2,
- Well 3 - a mixture of sewage from segments 1, 2 and 3,
- discharge outlet to drainage ditch.
Samples of raw sewage were taken from a well situated behind the accumulation -dispenser tank.
Chemical analyses of raw and treated sewage were carried out in the Institute of Environmental Studies and complied with the current norms. Bacteriological analyses of E. Coli numbers and coli titre were made at the Microbiology Department of Agricultural University in Wroclaw. Samples of raw and treated sewage for chemical and bacteriological tests were taken simultaneously. This was done twice, i.e. on Nov.9, 1997 and Dec.12, 1997. On Nov.9 air temperature was 6.2 oC, the temperature of raw sewage 11.6 oC and of treated sewage leaving the plant 9.6 oC, and on Dec.12 3.0 oC, 6.8 oC and 5.6 oC respectively. Bacteriological tests showed that the numbers of saprophytic E. Coli in raw sewage sampled at different times varied from 4× 106 to 4.5× 108 and from 3.8× 106 to 4× 108 for faecal E. Coli. Numbers of coliforms checked at different points of the plant were considerably decreased (Table 4). Numbers of faecal E. Coli in treated sewage leaving the plant changed from 140 to 1500, which translated into coli titre varied from 0.009 to 0.0007 (Table 5). A decreased value of coli titre was obtained for higher numbers of E. Coli in raw sewage. The analysis of test results from different parts of the plant shows considerable fluctuations in th numbers of E. Coli and in coli titre. Those changes were affected by the location of units which were fed with sewage right before the tests. The highest numbers of E. Coli in treated sewage occur in the first phase of sewage outflow after feeding the unit.
Table 4. Microbiological analysis of raw sewage and sewage treated in plant-soil treatment plant in Brzeźno (numbers of E. Coli/cm3) |
Place of sampling treated sewage |
Test date |
|||
09.11.1997 |
02.12.1997 |
|||
E. Coli |
E. Coli |
E. Coli |
E. Coli |
|
Raw sewage |
4 000 000 |
3 800 000 |
4.5·108 |
4.0·108 |
Well 3 |
900 |
400 |
95 |
95 |
Well 4 |
150 |
40 |
95 |
45 |
Well 5 |
1100 |
600 |
150 |
45 |
Discharge outlet |
200 |
140 |
11000 |
1500 |
Table 5. Coli titre for raw and treated sewage in plant soil treatment plant |
Place of sampling treated sewage |
Test date |
|||
09.11.1997 |
02.12.1997 |
|||
Coli titre |
Coli titre |
Coli titre |
Coli titre |
|
Raw sewage |
3·10-7 |
3·10-7 |
2·10-9 |
3·10-9 |
Well 3 |
0.0011 |
0.0025 |
0.0105 |
0.0105 |
Well 4 |
0.0067 |
0.025 |
0.0105 |
0.021 |
Well 5 |
0.0009 |
0.0017 |
0.0067 |
0.021 |
Discharge outlet |
0.005 |
0.007 |
0.0001 |
0.0007 |
Simultaneous to microbiological tests were studies of treatment efficiency for some chosen indexes of chemical contaminants. They showed that indexes for BOD5 and CODCr were very low in all segments of the plant including the discharge outlet of sewage treated in the tested plant (Table 6). Also analyses of treated sewage that included biogenic indicators, i.e. Kjeldahl nitrogen, ammonium nitrogen and phosphorus, showed that treated sewage contained insignificant amounts of those compounds that were much below the permissible values, Table 1 [12].
Table 6. Chemical analysis of raw and treated sewage in Brzeźno treatment plant |
Place of sampling treated sewage |
Test date |
|||
09.11.1997 |
02.12.1997 |
|||
BOD5 |
CODCr |
BOD5 |
CODCr |
|
mg O2/dm3 |
mg O2/dm3 |
|||
Raw sewage |
168.0 |
350.8 |
208.0 |
338.8 |
Well 3 |
0.6 |
10.6 |
3.9 |
12.3 |
Well 4 |
1.4 |
12.8 |
5.2 |
15.2 |
Well 5 |
0.8 |
9.4 |
3.9 |
12.7 |
Discharge outlet |
1.2 |
14.9 |
2.4 |
11.4 |
Results of raw and treated sewage analyses are shown in Tables 7 and 8. Table 8 additionally shows suspended solids in raw and treated sewage flowing out of different segments of the plant.
Table 7. Chemical analysis of raw and treated sewage in Brzeźno treatment plant (Kjeldahl nitrogen, ammonium nitrogen) |
Place of sampling treated sewage |
Test date |
|||
09.11.1997 |
02.12.1997 |
|||
Kjeldahl nitrogen |
Ammonium nitrogen |
Kjeldahl nitrogen |
Ammonium nitrogen |
|
mg N/dm3 |
mg N/dm3 |
|||
Raw sewage |
96.4 |
80 |
71.2 |
59.5 |
Well 3 |
1.5 |
1.04 |
1.65 |
0.59 |
Well 4 |
1.42 |
1.08 |
1.18 |
0.59 |
Well 5 |
1.2 |
0.94 |
2.55 |
0.66 |
Discharge outlet |
2.01 |
1.04 |
1.38 |
0.63 |
Table 8. Chemical analysis of raw and treated sewage in Brzeźno treatment plant (total phosphorus, suspended solids) |
Place of sampling treated sewage |
Test date |
||||||
09.11.1997 |
02.12.1997 |
||||||
Total phosphorus |
Suspended solids |
Total phosphorus |
Suspended solids |
||||
mg P/dm3 |
mg/dm3 |
mg P/dm3 |
mg/dm3 |
||||
Raw sewage |
12.5 |
288 |
11.8 |
102 |
|||
Well 3 |
0.17 |
68 |
0.16 |
30 |
|||
Well 4 |
0.16 |
19 |
0.17 |
12 |
|||
Well 5 |
0.23 |
70 |
0.12 |
24 |
|||
Discharge outlet |
0.14 |
46 |
0.06 |
8 |
DISCUSSION
Assessing microbiological efficiency of sewage treatment in a plant-soil treatment plant one should also analyse the efficiency of the chemical contaminants removal, without which the assessment may not be valid. Tests carried out in late autumn and early winter showed that the studied plant performed unususlly well in removing microbiological contaminants (coli titre). Treated sewage coming from different parts of the plant revealed very low numbers of E. Coli. Treatment efficiency resulting in lower numbers of saprophytic and faecal coliforms reached almost 100% (Table 9). The authors do not know of any sewage treatment plant that would be so efficient.
Table 9. Efficiency of microbiological treatment of wastewater (coli titre) in Brzeźno treatment plant [%] |
Place of sampling treated sewage |
Test date |
|||
09.11.1997 |
02.12.1997 |
|||
Coli titre |
Coli titre |
Coli titre |
Coli titre |
|
Well 3 |
99.973 |
99.988 |
99.99999 |
99.99999 |
Well 4 |
99.9955 |
99.9988 |
99.99999 |
99.9999 |
Well 5 |
99.9666 |
99.9824 |
99.9955 |
99.9995 |
Discharge outlet |
99.994 |
99.9957 |
99.921 |
99.9996 |
According to Boćko and Kutera [9] the highest reduction in E. Coli numbers can be obtained in constructed wetlands (Table 10).
Table 10. Decrease in bacteria in municipal wastewater after soil filtration [9] |
Place and way of irrigation |
Width of filtering soil stratum /cm/ |
Bacteria reduction % |
|
in total |
E. Coli |
||
Irrigation fields on sandy soil permanently flooded as lagoons |
120 |
95-97 |
99.9 |
Soil filters on loose sand permanently flooded |
120 |
90-99 |
99.9 |
Disposal fields on loose sand fed with high doses of sewage /500 mm/ |
120 |
85-99 |
99.0 |
Irrigation fields on clay with under-sand, highly-loaded |
120 |
94-99.6 |
99.9 |
Irrigation fields on different soils |
100 |
90-97 |
99-99.9 |
Irrigation fields on sandy soil fed with small doses |
100 |
99-99.9 |
99.99 |
Irrigation fields with sprinkling system on clay sand |
25 40 60 |
93-99 96-99.6 97-99.9 |
99.9 99.9 99.9 |
Bacteriological comparison of sewage treated in different parts of the plant (Table 11) makes it clear that as far as sanitary requirements are concerned it represents class 3 of water purity or slightly exceeds the borderline values for this class. Referring sewage treated in a plant-soil treatment plant to classes of water purity and considering indexes of aerobic contaminants and biogenic compounds one can conclude that for BOD5 treated sewage, independent of the part of the plant, represents class 1 with the purifying effect of 97.5% to 99.6% (Table 12). For CODCr sewage leaving the plant also represented class 1 and the purifying effect varied from 95.5% to 97%. The same was true for the Kjeldahl nitrogen content. Treatment efficienfy for this contamination index varied from 96.4% to 98.8% (Table 13). Ammonium nitrogen content was within permissible limits for classes 1 and 2. In comparison to r aw sewage, Kjeldahl nitrogen was lowered by 98.6% to 99%.
Table 11. Sewage effluent in Brzeźno treatment plant compared to classes of water purity |
Place of sampling |
Test date |
|||
09.11.1997 |
02.12.1997 |
|||
Coli titre |
Class of |
Coli titre |
Class of purity |
|
Well 3 |
0.0025 |
under class |
0.0105 |
III |
Well 4 |
0.025 |
III |
0.021 |
III |
Well 5 |
0.0017 |
under class |
0.021 |
III |
Discharge outlet |
0.007 |
under class |
0.0007 |
under class |
Receiving body of water |
0.0125 |
III |
- |
- |
Table 12. Efficiency of treatment for BOD5, CODCr [%] and class of water purity as represented by treated sewage |
Place of sampling treated sewage |
Test date |
|||||||
09.11.1997 |
02.12.1997 |
|||||||
BOD5 |
CODCr |
BOD5 |
CODCr |
|||||
class of purity |
η |
class of purity |
η |
class of purity |
η |
class of purity |
η |
|
Well 3 |
I |
99.6 |
I |
97.0 |
I |
98.1 |
I |
96.4 |
Well 4 |
I |
99.2 |
I |
96.4 |
I |
97.5 |
I |
95.5 |
Well 5 |
I |
99.5 |
I |
97.3 |
I |
98.1 |
I |
96.2 |
Discharge outlet |
I |
99.3 |
I |
95.8 |
I |
98.8 |
I |
96.6 |
Table 13. Efficiency of treatment for Kjeldahl nitrogen, ammonium nitrogen [%] and class of water purity as represented by treated sewage |
Place of sampling treated sewage |
Test date |
|||||||
09.11.1997 |
02.12.1997 |
|||||||
Kjeldahl nitrogen |
Ammonium nitrogen |
Kjeldahl nitrogen |
Ammonium nitrogen |
|||||
class of purity |
η |
class of purity |
η |
class of purity |
η |
class of purity |
η |
|
Well 3 |
I |
98.4 |
II |
98.7 |
I |
97.7 |
I |
99.0 |
Well 4 |
I |
98.5 |
II |
98.6 |
I |
98.4 |
I |
99.0 |
Well 5 |
I |
98.8 |
I |
98.8 |
I |
96.4 |
I |
98.9 |
Discharge outlet |
I |
97.8 |
II |
98.7 |
I |
98.1 |
I |
99.0 |
High efficiency of treatment was also observed for total phosphorus with a decrease of 98.2% to 99.5% and water purity was comparable to classes 1 and 2 (Table 14).
Table 14. Efficiency of treatment for total phosphorus, suspended solids [%] and class of water purity as represented by treated sewage |
Place of sampling treated sewage |
Test date |
|||||||
09.11.1997 |
02.12.1997 |
|||||||
Total phosphorus |
Suspended solids |
Total phosphorus |
Suspended solids |
|||||
class of purity |
η |
class of purity |
η |
class of purity |
η |
class of purity |
η |
|
Well 3 |
II |
98.6 |
under class |
76.4 |
II |
98.6 |
II |
70.6 |
Well 4 |
II |
98.7 |
I |
93.4 |
II |
98.6 |
I |
88.2 |
Well 5 |
II |
98.2 |
under class |
75.7 |
II |
99.0 |
II |
76.5 |
Discharge outlet |
II |
98.9 |
III |
84.1 |
I |
99.5 |
I |
92.2 |
Phosphorus content in effluent varied from 0.14 to 0.06 mg P/dm3.
Suspended solids in effluent complied with the requirements for classes 1 and 3. During the purification process the composition of suspended solids was changing. In raw sewage it was mostly of organic origin while in treated sewage mineral solids prevailed. Mineral suspended solids in treated sewage appeared mostly due to the washout of small particles from the soil.
The obtained results prove a very high efficiency of sewage treatment, which cannot be provided by treatment plants based on the active sludge or bacterial jelly methods. Treated sewage leaving the plant was comparable to water representing classes of purity from 1 to 3 with differences depending on the studied index. The numbers of E. Coli found in sewage treated in a plant-soil treatment plant can be obtained for sewage treated in a biological plant after exposure to UV.
Coliform numbers in treated sewage reach 104/cm3. Fig.2 shows a typical decrease in coliform numbers in the process of wastewater treatment [4].
Fig. 2. Standard lowering of coliforms in wastewater during treatment processes [4]
One can ask here how it happens that wastewater is so efficiently treated in a plant-soil treatment plant. Firstly, wastewater is treated in the active stratum of the soil. A large part of decomposed chemical compounds in sewage is absorbed by root systems of grass and trees overgrowing the treatment plant's area. Secondly, the surface stratum of the soil is 'inhabited' by a vast number of 'local' microorganisms whose number depends on such factors as way of cultivating, dampness, soil structure, chemism, etc. Once new microorganisms that are found in raw sewage get introduced, antagonisms between them and the local soil microorganisms begin. According to Balicka [3] this antagonism results from changes in environmental reaction, the range of anaero-andaerobiosis and the production of antibiotic substances which are a survival weapon in the fight between saprophytes and pathogenic species. There is also a mutual dependence between plants and the soil microflora. A con siderable growth of saprophytic microflora creates unfavourable conditions for the development of foreign microflora, e.g. in sewage. A very positive influence of rhizosphere on eliminating or preventing the growth of foreign microflora was also observed [1,2,3]. Studies by Balicka and Sobieszczański showed that an efficient retention of sewage microorganisms by the soil depends of the following:
- Depth of soil stratum,
- Soil structure,
- Mechanical composition,
- Amount and frequency of irrigation,
- Soil dampness.
According to Boćko [5], the number of bacteria in 1g of dry matter in a meadow not fed with sewage does not exceed 1 million, but doubles after irrigating it with a single dose of sewage (30mm) and exceeds 7 million at the depth of 20 cm after feeding a triple dose of 90 mm. In his studies of in-depth feeding of crops with wastewater Marcilonek [10] proved that coli titre in underground water is much higher (10-2 - 10-4) than in the sewage used for irrigation (10-5 - 10-6).
Studies by Boćko and Paluch [6] concerning the effect of sewage on underground water in the area of Osobowice wetlands in Wrocław showed no sanitary or chemical contamination. Available results of studies by foreign researchers connected with the elimination of microbiological contamination mostly refer to constructed wetlands with reed beds[7,11]. According to different sources, such treatment allows an average decrease in faecal coliforms from 95.3% to 98.9%.
The analysed research results acquired in a fully-operational treatment plant prove a very high efficiency of sewage treatment. Tests were carried out towards the end of the start-up period of the plant. One can ask about the efficiency of sewage treatment during further operation. It can only be expected that with time it will stay similar to that achieved in tests. As such type of a treatment plant is the first one built in this part of Poland further systematic studies are required. They should also answer the question of the influence which treatment plants like this have on the environment and on underground water in particular.
CONCLUSIONS
The following can be concluded from the research:
- A plant-soil treatment plant is highly efficient in lowering coli titre in treated sewage, which cannot be compared to any othertypes of treatment plants.
- A very high microbiological efficienfy of sewage treatment was also reflected in an unusual lowering of aerobic and biogenic indexes.
- The studies should be continued. This would allow, among other things, to assess the influence of the treatment plant on the environment, including underground water situated below it.
ACKNOWLEDGEMENTS
The authors wish to express their gratitude to the authorities of Agricultural University in Wrocław and the deans of the Faculty of Land Improvement and Environmental Engineering whose understanding and financial support resulted in the practical realisation of Project 202/GW/97 and made this publication possible.
BIBLIOGRAPHY
Submited: 12.05.1998
Krzysztof Kuczewski, Joanna Kercel-Cwalińska, Edmund Solecki, Iwona Nowak
Institute of Agricultural Building,
Agricultural University
Pl. Grunwaldzki 24, 50-363 Wrocław, Poland
Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed Discussions in each series and hyperlinked to the article.