Electronic Journal of Polish Agricultural Universities (EJPAU) founded by
all Polish agriculture universities presents original papers and review articles
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- UTP University of Science and Technology in Bydgoszcz
- University of Agriculture in Krakow
- University of Life Sciences in Lublin
- University of Warmia and Mazury in Olsztyn
- Poznań University of Life Sciences
- Siedlce University of Natural Sciences and Humanities
- West Pomeranian University of Technology Szczecin
- Warsaw University of Life Sciences - SGGW
- Wroc³aw University of Environmental and Life Sciences
Founded in 1998 ISSN 1505-0297
Issue 21 (4) #7
authors:
title: ASSESSMENT OF METAL NANOPARTICLE-INDUCED MORPHOLOGICAL AND PHYSIOLOGICAL CHANGES IN IN VITRO CULTURES OF RAPESEED (BRASSICA NAPUS L.)
DOI:10.30825/5.EJPAU.166.2018.21.4
keywords: catalase, chlorophyll, nanocopper, nanogold, nanosilver, oxidative stress
abstract: To understand the mechanisms involved in plant responses to metal nanoparticles, the morphological and physiological changes in rapeseed plants (Brassica napus L.) were determined. The sterile seeds were inoculated onto the MS medium (sterilized and unsterilized) containing silver, copper and gold nanoparticles at the concentration of 20 ppm. Silver nanoparticled did not inhibit the germination process as opposed to cooper nanoparticles. The nanoparticles did not affect the roots length. The seedlings grown on the medium supplemented with nanoparticles were significantly shorter in comparison to the control. Nanosilver without autoclave sterilization reduced the plant height. The total chlorophyll, the ratio of chl a / chl b and carotenoid content decreased after the exposure to metal nanoparticles. Additionally, it was found that the nanoparticles of gold increased the activity of catalase, as compared with the control. There is a growing number of research of the biological effects of nanoparticles on higher plants. The application of nanoparticles in in vitro cultures can be very important to facilitate understanding the nanoparticles toxicity for plants.
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