Electronic Journal of Polish Agricultural Universities (EJPAU) founded by
all Polish agriculture universities presents original papers and review articles
relevant to all aspects of natural and engineering sciences. Covered by AGRICOLA,
AGRO, AGRIS, Copernicus Index, IFIS Publishing (FSTA – Food Science and
Technology Abstracts), DOJE-Directory of Open Access Journal, National Marine
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in the Master List of Thomson ISI. License CC-BY.
- UTP University of Science and Technology in Bydgoszcz
- University of Agriculture in Krakow
- University of Life Sciences in Lublin
- University of Warmia and Mazury in Olsztyn
- Poznań University of Life Sciences
- Siedlce University of Natural Sciences and Humanities
- West Pomeranian University of Technology Szczecin
- Warsaw University of Life Sciences - SGGW
- Wroc³aw University of Environmental and Life Sciences
Founded in 1998 ISSN 1505-0297
Issue 19 (4) #9
authors:
title: FAST MOLECULAR DETECTION OF PECTOBACTERIUM ATROSEPTICUM, PECTOBACTERIUM CAROTOVORUM SUBSP. CAROTOVORUM, AND DICKEYA SPP. FROM POTATO (SOLANUM TUBEROSUM L.) STEM TISSUES
keywords: PCR detection, specific primers, potato plant wilting, pectinolytic Enterobacteriaceae
abstract: Detection of the pathogenic bacteria causing blackleg of potato plants is important for seed producers, growers and potato industry. The conventional PCR method using primers specific for Pectobacterium atrosepticum (Y45/Y46), Pectobacterium carotovorum subsp. carotovorum (together with Pectobacterium wasabiae) (INPCCF/INPCCR) and Dickeya spp. (Ech1/Ech1’) turned out to be useful to determine the presence of these bacteria among the various microorganisms inhabiting potato stems. In order to minimize the time and costs of a commercial analysis, the pathogen detection method omitted bacterial DNA extraction step. Moreover, universal organic medium was applied for enrichment of the microbial cell number to detection level instead of commonly used isolation of pure bacterial cultures on selective media. Results of our studies confirmed the rising problem of the infection with Dickeya spp. and P. carotovorum subsp. carotovorum which caused the potato plant wilting during hot summer.
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