Issue 19 (4) #9

authors:

title: FAST MOLECULAR DETECTION OF PECTOBACTERIUM ATROSEPTICUM, PECTOBACTERIUM CAROTOVORUM SUBSP. CAROTOVORUM, AND DICKEYA SPP. FROM POTATO (SOLANUM TUBEROSUM L.) STEM TISSUES

keywords: PCR detection, specific primers, potato plant wilting, pectinolytic Enterobacteriaceae

abstract: Detection of the pathogenic bacteria causing blackleg of potato plants is important for seed producers, growers and potato industry. The conventional PCR method using primers specific for Pectobacterium atrosepticum (Y45/Y46), Pectobacterium carotovorum subsp. carotovorum (together with Pectobacterium wasabiae) (INPCCF/INPCCR) and Dickeya spp. (Ech1/Ech1’) turned out to be useful to determine the presence of these bacteria among the various microorganisms inhabiting potato stems. In order to minimize the time and costs of a commercial analysis, the pathogen detection method omitted bacterial DNA extraction step. Moreover, universal organic medium was applied for enrichment of the microbial cell number to detection level instead of commonly used isolation of pure bacterial cultures on selective media. Results of our studies confirmed the rising problem of the infection with Dickeya spp. and P. carotovorum subsp. carotovorum which caused the potato plant wilting during hot summer.

All time count: 436 article

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