abstract: Pot experiment on yellow lupine cv. Mister covered the differentiated N_min content in plant growth medium and methods of seed preparation for sowing, including seed inoculation with Bradyrhizobium lupini and/or genistein. Abundance of Nmin in the growth substrate as well as method of preparing seeds for sowing did not affect the formation of the mean dry matter of yellow lupine plants and their total N content. The highest dry matter of seeds per one lupine plant was obtained with a high (calculated for field conditions 80 kg N·ha^-1) N_min content in the growth substrate. The total N content in the aboveground parts of plants significantly decreased since 35 days after emergence, and was significantly the lowest at full seed maturity, while in the underground parts the total N underwent significantly smaller changes during vegetation. The mean N content in yellow lupine seeds did not significantly depend on the abundance of Nmin in the growth substrate, nor on the use of seed inoculation with rhizobia and genistein.