Volume 8
Issue 2
Food Science and Technology
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Available Online: http://www.ejpau.media.pl/volume8/issue2/abs-12.html
EFFECT OF SELECTED LIPID SUBSTRATES ON THE PROCESS OF BIOSYNTHESIS OF SURFACE-ACTIVE COMPOUNDS BY THE CANDIDA BOMBICOLA YEASTS
Małgorzata Gumienna, Maria Czarnecka, Zbigniew Czarnecki
Department of Fermentation and Biosynthesis,
Institute of Food Technology of Plant Origin,
The August Cieszkowski Agricultural University of Poznan, Poland
ABSTRACT
The performed investigations were concerned with the possibility of utilisation of the yeasts ATCC 22214 Candida bombicola for the biosynthesis of surface active substances on substrates containing oleic acid, sunflower oil and post-deodorising condensate as carbon sources. Glucose was the source of hydrophilic carbon in the medium, whereas nitrogen was supplied by the yeast extract. The highest yields of biosurfactants (124.5 g·dm-3 - oleic acid, 116 g·dm-3 - sunflower oil and 118.5 g·dm-3 - post-deodorising condensate) were achieved using the medium in which 3 g of glucose and 0.05 g of yeast extract and 1 g of the fat substrate. The obtained biosurfactants, depending on the fat substrate applied in the culture medium, differed with respect to their appearance. The obtained preparations reduced the surface tension of water from 70 mN/m to approximately 32 mN/m.
Key words: biosurfactants, biosynthesis, Candida bombicola, fat substrate.
Małgorzata Gumienna
Department of Fermentation and Biosynthesis,
Institute of Food Technology of Plant Origin,
The August Cieszkowski Agricultural University of Poznan, Poland
Wojska Polskiego 31, 60-624 Poznan, Poland
ph: (+4861) 848 72 67
email: e-mail:gumienna@au.poznan.pl
Maria Czarnecka
Department of Fermentation and Biosynthesis,
Institute of Food Technology of Plant Origin,
The August Cieszkowski Agricultural University of Poznan, Poland
Wojska Polskiego 31, 60-624 Poznan, Poland
Zbigniew Czarnecki
Department of Fermentation and Biosynthesis,
Institute of Food Technology of Plant Origin,
The August Cieszkowski Agricultural University of Poznan, Poland
Wojska Polskiego 31, 60-624 Poznan, Poland
email: zbyczar@au.poznan.pl
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