Volume 19
Issue 4
Horticulture
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Available Online: http://www.ejpau.media.pl/volume19/issue4/abs-02.html
LEDS APPLICATION IN EX VITRO ROOTING AND ACCLIMATIZATION OF CHRYSANTHEMUM (CHRYSANTHEMUM X GRANDIFLORUM /RAMAT./ KITAM.)
Anita Wo¼ny, Natalia Miler
Department of Ornamental Plants and Vegetable Crops, Faculty of Agriculture and Biotechnology, University of Science and Technology, Bydgoszcz, Poland
ABSTRACT
Plants of four chrysanthemum cultivars propagated in vitro were tested in terms of survival and plantlets quality after acclimatization under fluorescent lamps (FLs) and light-emitting diodes (LEDs). The plantlets were acclimatized directly or after in vitro rooting. Two out of four cultivars showed lowered survival capability after direct acclimatization; only a few plantlets of ‘Bingo’ and ‘Cool Time’ survived, while most plantlets representing ‘Euro’ and ‘Vymini’ were properly acclimatized, rooted and growing. The survival rate in plantlets acclimatized after in vitro rooting was high, from 85–95% in all the cultivars. The light source did not influence the survival rates. Plantlets quality after acclimatization under LEDs or FLs differed in particular cultivars. In ‘Bingo’ and ‘Vymini’ acclimatized under LEDs the average height of plantlets and fresh weight was higher than under FL. The length of roots was only affected by light source in ‘Vymini’; the roots produced under LEDs were longer. Chlorophyll content was higher under LEDs in ‘Cool Time’ and ‘Euro’. Chrysanthemum acclimatization can be successfully conducted under LEDs and FLs, however it should be forgone by in vitro rooting.
Key words: direct rooting, light-emitting diodes, micropropagation.
Anita Wo¼ny
Department of Ornamental Plants and Vegetable Crops, Faculty of Agriculture and Biotechnology, University of Science and Technology, Bydgoszcz, Poland
Bernardyńska 6/8, 85-029 Bydgoszcz, Poland
email: wozny@utp.edu.pl
Natalia Miler
Department of Ornamental Plants and Vegetable Crops, Faculty of Agriculture and Biotechnology, University of Science and Technology, Bydgoszcz, Poland
Bernardyńska 6, 85-029 Bydgoszcz, Poland
Phone: (+48) 52 374 95 22
email: nmiler@utp.edu.pl
Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.