EJPAU 2010. Kanownik W. , Rajda W. QUALITY INDICES OF WATERS FLOWING AWAY FROM CATCHMENTS OF SMALL RETENTION RESERVOIRS PLANNED IN THE KRAKOW REGION

Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

2010
Volume 13
Issue 3

Topic:

Environmental Development

ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES

Kanownik W. , Rajda W. 2010. QUALITY INDICES OF WATERS FLOWING AWAY FROM CATCHMENTS OF SMALL RETENTION RESERVOIRS PLANNED IN THE KRAKOW REGION, EJPAU 13(3), #08.
Available Online: http://www.ejpau.media.pl/volume13/issue3/abs-08.html

QUALITY INDICES OF WATERS FLOWING AWAY FROM CATCHMENTS OF SMALL RETENTION RESERVOIRS PLANNED IN THE KRAKOW REGION

Włodzimierz Kanownik, Włodzimierz Rajda
Department of Land Reclamation and Environmental Development, University of Agriculture, Cracow, Poland

ABSTRACT

Flow cytometry (FC) was used for measuring ploidity and number of cells at each phase of the cell cycle of four Yarrowia lipolytica yeast strains. Synchronization at G0/G1-phase was partially achieved by carbon source starvation, followed by glucose addition. Approximately 10 000 cells were analyzed by measuring the fluorescence of propidium iodine (PI) bound to DNA after 0, 1, 2, 4, 6 hours of glucose addition to the starved cells population. Estimation of cells percentage at each cycle phase was achieved by Cylchred and WinMDI analysis of obtained data. However complete synchronization was not obtained for any of the strains, the generation time representing time interval between two maxima of cells at S phase was determined. The shortest generation time was noted for Y. lipolytica AWG7 (1h).

Comparison of histograms of all tested strains with a haploid type strain proved their haploidy.

Key words: small retention, catchment, anthropogenic factors, water, quality.

Włodzimierz Kanownik
Department of Land Reclamation and Environmental Development,
University of Agriculture, Cracow, Poland
al. Mickiewicza 24/28, 30-059 Cracow, Poland
email: rmkanown@cyf-kr.edu.pl

Włodzimierz Rajda
Department of Land Reclamation and Environmental Development,
University of Agriculture, Cracow, Poland
al. Mickiewicza 24/28, 30-059 Cracow, Poland
email: rmrajda@cyf-kr.edu.pl

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