Volume 7
Issue 2
Biotechnology
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Available Online: http://www.ejpau.media.pl/volume7/issue2/biotechnology/abs-01.html
COMPARISON OF DIFFERENT STRAINS OF ASPERGILLUS NIGER FOR OXALIC ACID PRODUCTION FROM LIPID SUBSTRATES
Waldemar Rymowicz, Dorota Lenart
ABSTRACT
One wild-type strain and eight mutants of the fungi species of Aspergillus niger were screened for their ability to produce oxalic acid on lipids media using batch fermentation. All of the tested fungi strains were able to utilize crude rapeseed oil as energy and carbon source. A final oxalic acid concentration ranged from 8 to 66.1 g/L, depending on the strain used. A. niger XP (induced with UV irradiation) was found to be the most suitable for oxalic acid production from lipid substrates. The highest production of oxalic acid (66.1 g/L), the oxalate yield (1.39 g/g) and the overall oxalate productivity (9.4 g/L·d) were obtained when the fermentation medium contained 50 g/L of oil and at pH 5 maintained from the beginning of the fermentation process.
Key words: Aspergillus niger, oxalic acid, biosynthesis, lipids.