Volume 7
Issue 1
Horticulture
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Available Online: http://www.ejpau.media.pl/volume7/issue1/horticulture/abs-04.html
THE EFFECT OF FUNGICIDES APPLIED IN APPLE ORCHARDS ON ENTOMOPATHOGENIC FUNGI IN VITRO
Anna Sapieha-Waszkiewicz, Barbara Marjańska-Cichoń, Ryszard Miętkiewski
ABSTRACT
The experiment tested the following entomopathogenic fungi isolated by the method of bait insects: Beauveria bassiana (Bb), Metarhizium anisopliae (Ma) and Paecilomyces fumosoroseus (Pf), which were obtained from a cultivated field (Bb I, Ma I, Pf I) and from a herbicide fallow belt (Bb II, Ma II, Pf II) in an apple orchard with a full program of plant protection. The fungi were inoculated on Sabouraud’s medium with an addition of fungicides: Atemi C 76 WG, Delan 700 WG, Captan 50 WP and Rubigan 12 EC in the following doses: one recommended in agricultural practice – (A), 10 times lower than the recommended one – (B) and 100 times lower than the recommended one (C). The studies showed that the effect of fungicides on entomopathogenic fungi was related to the active substance of the preparations while the toxic effect was most frequently directly proportional to their concentration in the soil. Fungus M. anisopliae was the most sensitive to the applied
Key words: fungicides, entomopathogenic fungi, growth and morphology of colonies, germination of spores.