Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

Copyright © Wydawnictwo Akademii Rolniczej we Wroclawiu, ISSN 1505-0297

Zdunek A. , Umeda M. , Konstankiewicz K. 2004. METHOD OF PARENCHYMA CELLS PARAMETRISATION USING FLUORESCENCE IMAGES OBTAINED BY CONFOCAL SCANNING LASER MICROSCOPE, EJPAU 7(1), #01.
Available Online: http://www.ejpau.media.pl/volume7/issue1/engineering/abs-01.html

ABSTRACT

Fruits and vegetables are built mainly of thin walled parenchyma cells highly susceptible to mechanical damage. According to experimental investigation of relationship between cells geometrical parameters and mechanical properties of tissue a new efficient method of structure reconstruction and parametrisation is elaborated and presented in this paper. Special sample preparation and way of image taking by Confocal Scanning Laser Microscope with fluorescence mode allows obtaining images of any number of cells. Unique computer procedure based on morphological operators was created that allows analysing automatically any number of images with extracting geometrical parameters of each cell separately. The method was tested on potato and carrot tissue. Results show that the method is fast and gives proper cell reconstruction for both materials. In order to decrease error of incorrect reconstruction, possibility of manual correction was introduced to the procedure.

Key words: microscope image, image analysis, cell size, cell shape, plant tissue.

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