Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

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Chorbiński P. , Rypuła K. 2003. STUDIES ON THE MORPHOLOGY OF STRAINS ASCOSPHAERA APIS ISOLATED FROM CHALKBROOD DISEASE OF THE HONEY BEES, EJPAU 6(2), #05.
Available Online: http://www.ejpau.media.pl/volume6/issue2/veterinary/abs-05.html

ABSTRACT

The Ascosphaera genus (Plectomycetes: Ascosphaeraceae) is found in the environment solely in populations of social and solitary bees. A typical feature of the genus Ascosphaera is production of spherical spore cysts abundant in ascospores forming spore balls. The species vary in the size of spore cysts, as well as in the shape and size of ascospores. The aim of this study was to determine morphological properties of strains A. apis isolated from field cases of chalkbrood diseased honeybees. The study material was inoculated onto Sabouarda’s medium, underwent macroscopical and microscopical identification and was examined for morphological details by cryo-electron microscopy with a scanning electron microscope. The examination results and measurements obtained with the scanning microscope enable us to confirm the results received by means of commonly applied diagnostic techniques to determine whether the strains under study belong to the genus Ascosphaera a

Key words: Ascosphaera apis, honey bee, brood cells, scanning electron microscope..

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