Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

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Błażejak S. , Duszkiewicz-Reinhard W. , Gniewosz M. , Górski K. 2003. AN ATTEMPT TO LOCALISE MAGNESIUM IN THE CELLS OF BAKER’S YEASTS SACCHAROMYCES CEREVISIAE NO. 102 SUPPLEMENTED WITH THIS ELEMENT, EJPAU 6(2), #08.
Available Online: http://www.ejpau.media.pl/volume6/issue2/food/abs-08.html

ABSTRACT

The study aimed at determining which part of magnesium permanently bound with baker’s yeasts S. cerevisiae No. 102 during batch culture in control (YPD) and experimental medium (YPD with the addition of magnesium) remains in the cell wall and which undergoes intracellular bio-accumulation. The experimental media were supplemented with the amount of MgCl₂*6H₂O providing 1.25 g/dm³ content of Mg²⁺ ions. The cultures were run for 24 h at 28°C in a reciprocating shaker which provided aerobic conditions of the process. The application of enzymatic protoplastisation and mechanical disintegration of the yeast cells (at a temperature of liquid nitrogen) enabled determination of the contents of magnesium and total protein in both cell walls and protoplasts. The yeasts from a 24 h batch culture in the control YPD medium (without magnesium) demonstrated diversified contents of magnesium in the cell walls (1.84 mg Mg²⁺/g d.m. of

Key words: bioplexes, metalloproteins, magnesium, bio-elements, Saccharomyces cerevisiae..

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