Volume 6
Issue 2
Environmental Development
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Available Online: http://www.ejpau.media.pl/volume6/issue2/environment/abs-05.html
FOSPHATASE ACTIVITY AND ATP CONTENT IN SOIL AND PLANT RELATED TO COPPER (II) NITRATE (V) CONCENTRATION IN SOIL
Janina Nowak, Bożena Śnieg, Justyna Szymczak
ABSTRACT
The paper presents the results of a pot experiment on phosphatase activity and ATP level in plant and in soil induced by diversified doses of copper (II) nitrate (V). Five combinations of copper (II) salt were applied; each combination was conducted in 4 identical series. Water solutions of copper (II) nitrate (V) were introduced into 5 soil samples in the following doses: no salt introduced (control), 0.05 mmol. kg-1, 0.50 mmol·kg-1, 5.00 mmol·kg-1, 50.00 mmol.kg-1 of soil for the samples from 1 to 5, respectively. Size grading of the soil experimented was typical for light silty clay. Sown Pea /Pisum sativum L./ of Amethyst variety served as a test plant. Enzymes activities and ATP contents in both soil and plant samples were analysed in various developmental stages of Sown Pea, namely at the two-leaves, blooming and green legume stages. Copper (II) nitrate (V) introduced into soil (apart from 0.0
Key words: asoil, phosphatase activity, ATP..