Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2003
Volume 6
Issue 2
Topic:
Biology
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Chrzanowski G. , Ciepiela A. , Sprawka I. , Sempruch C. , Sytykiewicz H. , Czerniewicz P. 2003. ACTIVITY OF POLYPHENOLOXIDASE IN THE EARS OF SPRING WHEAT AND TRITICALE INFESTED BY GRAIN APHID (Sitobion avenae /F./), EJPAU 6(2), #04.
Available Online: http://www.ejpau.media.pl/volume6/issue2/biology/abs-04.html

ACTIVITY OF POLYPHENOLOXIDASE IN THE EARS OF SPRING WHEAT AND TRITICALE INFESTED BY GRAIN APHID (SITOBION AVENAE /F./)

Grzegorz Chrzanowski, Antoni P. Ciepiela, Iwona Sprawka, Cezary Sempruch, Hubert Sytykiewicz, Pawe│ Czerniewicz

 

ABSTRACT



Polyphenoloxidase (PPO) plays an important role in plant resistance to insects, and in the detoxication of phenolic compounds taken in the nutrient components. Activity of PPO was examined in the ears of spring wheat and triticale. Extraction of enzymatic protein was carried out from acetone powder with the use of 0.05M phosphoric buffer of pH 7.4. The cultivars of spring wheat have possessed higher activity of PPO than cultivars of triticale. Feeding of the grain aphid has reduced this enzyme activity in the all analysed species and cultivars.

Key words: polyphenoloxidase, spring wheat, spring triticale, Sitobion avenae..