Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2003
Volume 6
Issue 2
Topic:
Animal Husbandry
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Brodacki A. , Tarkowski J. , Flis J. 2003. GENETIC DISTANCES IN HENS ESTIMATED WITH PROTEIN GENES FREQUENCIES AND PROCEDURES OF DNA ANALYSIS, EJPAU 6(2), #08.
Available Online: http://www.ejpau.media.pl/volume6/issue2/animal/abs-08.html

GENETIC DISTANCES IN HENS ESTIMATED WITH PROTEIN GENES FREQUENCIES AND PROCEDURES OF DNA ANALYSIS

Antoni Brodacki, Janusz Tarkowski, Jaros│aw Flis

 

ABSTRACT



Egg yolk and white proteins from hens kept in eight conservation flocks in Poland were separated with horizontal polyacrylamide gel electrophoresis. The analyses were done on 100-120-egg samples from each of the following breeds: Green-Legged Partridge, ZKF and ZK11, Yellow-Legged Partridge, Z33, Polbar, PB, Leghorn, H22 and G99, Rhode Island Red, RD2, and Sussex, S66. After estimating phenotype frequency based on 30-35 bands on the electrophoregrams, the following were identified: gene frequencies of fast-migrating prealbumins, Pa-F, and transferrins, TF, in egg yolk, as well as ovalbumins Ov-A, ovoglobulins, G3, G4, G2, and conalbumins, Co, in the white of egg. Similarity and Nei's genetic distances were calcula-ted according to gene frequency (GF) and procedures used in analysing DNA polymorphism, based on frequencies of particular protein bands (BF) and band sharing (BS). Application of GF and BF in the evaluation of genetic distances gave similar results. The shortest distances were n

Key words: protein polymorphism, genetic distance, chickens..