Volume 6/issue1/volume6
Issue 1
Biotechnology
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Available Online: http://www.ejpau.media.pl/volume6/issue1/volume6/issue1/biotechnology/abs-01.html
SELECTED BIOTECHNOLOGICAL FEATURES OF HYBRIDS OF SACCHAROMYCES CEREVISIAE AND YAMADAZYMA STIPITIS
Joanna Chmielewska
ABSTRACT
The aim of the research was to investigate the possibility of the use of interspecific hybrids of yeast in ethanol fermentation of media containing xylose. Distilling yeast Saccharomyces cerevisiae D43, xylose fermenting yeast Yamadazyma stipitis ATCC 58376 and their hybrids: YD43-4, YD43-6, YD43-11 and YD43-12 were used as biological material. The parental strain Yamadazyma stipitis ATCC 58376 produced 0.389 g ethanol from 1 g xylose. The hybrids YD43-6 and YD43-11 did not statistically differ from Yamadazyma stipitis in respect to efficiency of ethanol from xylose, but produced less xylitol. In the model medium, with the ratio of glucose to xylose (7:3) corresponding to lignocellulitic hydrolysates, the hybrid YD43-6 produced the most ethanol (0.377 g/g).
Key words: distilling, distilling yeast, protoplast fusion, xylose fermentation.