Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2003
Volume 6
Issue 1
Topic:
Food Science and Technology
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Leśnierowski G. , Kijowski J. , Stangierski J. 2003. DSC, SDS-PAGE AND SPECTROPHOTOMETRY FOR CHARACTERIZATION OF MODIFIED LYSOZYME, EJPAU 6(1), #11.
Available Online: http://www.ejpau.media.pl/volume6/issue1/food/abs-11.html

DSC, SDS-PAGE AND SPECTROPHOTOMETRY FOR CHARACTERIZATION OF MODIFIED LYSOZYME

Grzegorz Leśnierowski, Jacek Kijowski, Jerzy Stangierski

 

ABSTRACT



The aim of the study was an attempt to apply selected analytical methods for the evaluation of preparations of modified lysozyme. Lysozyme isolated from hen egg white was modified using thermal, thermal-chemical, chemical and membrane methods and subsequently the obtained preparations were evaluated with the use of the spectrophotometric method, as well as electrophoresis and differential scanning calorimetry (DSC).

Key words: differential scanning calorimetry (DSC), spectrophotometry, electrophoresis, lysozyme modification, lysozyme dimer, hydrolytic activity.