Available Online: http://www.ejpau.media.pl/volume5/issue2/veterinary/abs-01.html
INFLUENCE OF DIMERIZED LYSOZYME (KLP-602) ON THE IMMUNE RESPONSES INDUCED BY FUROGEN VACCINE IN RAINBOW TROUT (ONCORHYNCHUS MYKISS)
Andrzej K. Siwicki, Peter Klein, Krzysztof Kazuń, Marc Morand, Edward Gł±bski, Sylwia Trapkowska
In the present study the possible stimulation of nonspecific and specific immune response after immunization induced by immersion Furogen (Aqua Health Ltd) vaccine were analysed in rainbow trout (Oncorhynchus mykiss). The modulation was attempted using a natural immunostimulant, dimerized lysozyme (KLP-602). Five groups of rainbow trout were given intraperitoneal injections of KLP-602 two days before vaccine, with vaccine, and two days after vaccine applicated by immersion. The results of cellular study showed that KLP-602 applicated before or with Furogen vaccine statistically significant (P<0.05) increased the total immunoglobulin secreting cells (ISC) and specific antibody secreting cells (ASC) levels, compared to the KLP-free (only vaccinated) group of fish. Analysis of the results shows no significant differences between group of fish where KLP-602 was applicated after vaccination and KLP-free (only vaccinated) group of fish. Analysis the results of humoral study shows that KLP-602 applicated
Key words: Lysozyme dimer, fish, Furogen vaccine, ASC, specific immune response.