Available Online: http://www.ejpau.media.pl/volume5/issue2/forestry/abs-02.html
THE ESTIMATION OF GENETIC VARIATION WITHIN AND BETWEEN POLISH PROVENANCES OF NORWAY SPRUCE (PICEA ABIES (L.) KARST.) ON THE BASIS OF RAPD POLYMORPHISM
In order to determine the genetic variation of Norway spruce (Picea abies (L.) Karst.) investigations on the polymorphism of the genomic DNA were conducted. The DNA was extracted from needles of trees belonging to 20 Polish provenances of Norway spruce. For the analysis of DNA the RAPD method with four 10- and 11-nucleotide primers was employed. In the pilot study the optimal concentration of magnesium ions used for the PCR reaction was determined. That allowed for obtaining a restricted right number of discrete and easily identifiable bands. In total, 45 products of amplification were obtained; they were further used for determining the variation of the analysed provenances. The usefulness of RAPD method for determining the inter- and intrapopulation genetic variation in Norway spruce was demonstrated. In this study the characteristic for each population monomorphic bands, allowing to identify each population, were obtained. The similarities of individuals within population (based on the Jaccard's coe
Key words: Picea abies, genetic markers, RAPD, genetic diversity, identification.