Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

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Kraj W. 2002. THE ESTIMATION OF GENETIC VARIATION WITHIN AND BETWEEN POLISH PROVENANCES OF NORWAY SPRUCE (PICEA ABIES (L.) KARST.) ON THE BASIS OF RAPD POLYMORPHISM, EJPAU 5(2), #02.
Available Online: http://www.ejpau.media.pl/volume5/issue2/forestry/abs-02.html

ABSTRACT

In order to determine the genetic variation of Norway spruce (Picea abies (L.) Karst.) investigations on the polymorphism of the genomic DNA were conducted. The DNA was extracted from needles of trees belonging to 20 Polish provenances of Norway spruce. For the analysis of DNA the RAPD method with four 10- and 11-nucleotide primers was employed. In the pilot study the optimal concentration of magnesium ions used for the PCR reaction was determined. That allowed for obtaining a restricted right number of discrete and easily identifiable bands. In total, 45 products of amplification were obtained; they were further used for determining the variation of the analysed provenances. The usefulness of RAPD method for determining the inter- and intrapopulation genetic variation in Norway spruce was demonstrated. In this study the characteristic for each population monomorphic bands, allowing to identify each population, were obtained. The similarities of individuals within population (based on the Jaccard's coe

Key words: Picea abies, genetic markers, RAPD, genetic diversity, identification.

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