Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2002
Volume 5
Issue 2
Topic:
Biotechnology
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Maj A. , Witkowska D. , Robak M. 2002. BIOSYNTHESIS AND PROPERTIES OF beta-1,3-GLUCANASES OF Trichoderma hamatum, EJPAU 5(2), #01.
Available Online: http://www.ejpau.media.pl/volume5/issue2/biotechnology/abs-01.html

BIOSYNTHESIS AND PROPERTIES OF BETA-1,3-GLUCANASES OF TRICHODERMA HAMATUM

Agnieszka Maj, Danuta Witkowska, Ma│gorzata Robak

 

ABSTRACT



The strain of Trichoderma hamatum C-1 is able to produce extracellular lytic enzymes i.e. ▀-1,3- glucanases, chitinases, ▀-1,6-glucanases in the shaked cultures in the base medium enriched with fodder yeast supplemented with Avicel cellulose, as well as with glucose. Glucose had a repressive effect on ▀-1,3- glucanase activity only when the fungus was cultivated in a high quantity (3%) of glucose medium. The enzymes were precipitated from liquid culture medium by ethanol and then optimal conditions for the enzymatic activity (pH 3.0-5.0; 50°C) and stability (pH 5.0; 50°C) of ▀-1,3-glucanases were determined. The enzyme activity was strongly inhibited in the presence of HgCl2 , EDTA and stimulated by cations such as Mg+2 and Ca+2. After enzymatic hydrolysis (120 min.)of laminarin products of reaction were measured using HPLC and about 90% of glucose and a little laminaribiose and laminaritriose were obtained. The enzymatic preparation was used for Yarrowi

Key words: lytic anzymes, beta-1,3-glucanases, biosynthesis, biochemical properties, Trichoderma hamatum.