Available Online: http://www.ejpau.media.pl/volume5/issue2/biotechnology/abs-01.html
BIOSYNTHESIS AND PROPERTIES OF BETA-1,3-GLUCANASES OF TRICHODERMA HAMATUM
Agnieszka Maj, Danuta Witkowska, Ma│gorzata Robak
The strain of Trichoderma hamatum C-1 is able to produce extracellular lytic enzymes i.e. ▀-1,3- glucanases, chitinases, ▀-1,6-glucanases in the shaked cultures in the base medium enriched with fodder yeast supplemented with Avicel cellulose, as well as with glucose. Glucose had a repressive effect on ▀-1,3- glucanase activity only when the fungus was cultivated in a high quantity (3%) of glucose medium. The enzymes were precipitated from liquid culture medium by ethanol and then optimal conditions for the enzymatic activity (pH 3.0-5.0; 50°C) and stability (pH 5.0; 50°C) of ▀-1,3-glucanases were determined. The enzyme activity was strongly inhibited in the presence of HgCl2 , EDTA and stimulated by cations such as Mg+2 and Ca+2. After enzymatic hydrolysis (120 min.)of laminarin products of reaction were measured using HPLC and about 90% of glucose and a little laminaribiose and laminaritriose were obtained. The enzymatic preparation was used for Yarrowi
Key words: lytic anzymes, beta-1,3-glucanases, biosynthesis, biochemical properties, Trichoderma hamatum.