Volume 5/issue1/volume5
Issue 1
Horticulture
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Available Online: http://www.ejpau.media.pl/volume5/issue1/volume5/issue1/horticulture/abs-02.html
THE APPLICATION OF RAPD MARKERS FOR GENETIC STUDIES ON FRAGARIA X ANANASSA DUCH. (STRAWBERRY) WITH DUE CONSIDERATION FOR PERMANENT FLOWERING
Elżbieta Kaczmarska, Jerzy A. Hortyński
ABSTRACT
The level of genetic diversity between 16 cultivars of strawberry (Fragaria x ananassa Duch.) and the wild species of Fragaria virginiana Duch. was studied on the basis of the analysis of their DNA (RAPD reaction – randomly amplified polymorphic DNA). Six 10-nucleotide primers generated jointly 354 bands, of which 94.8% were polymorphic and 5.2% monomorphic. The analysis of interrelations was carried out on the basis of Dice similarity matrix. Presentation of relationship between the analysed genotypes on the dendrogram obtained by means of the unweighted pair-group method with arithmetic average (UPGMA).
Key words: bulked segregant analysis (BSA), photoperiodism, Fragaria x ananassa, genetic similarity, RAPD markers.