Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2002
Volume 5
Issue 1
Topic:
Horticulture
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Kaczmarska E. , Hortyński J. 2002. THE APPLICATION OF RAPD MARKERS FOR GENETIC STUDIES ON FRAGARIA X ANANASSA DUCH. (STRAWBERRY) WITH DUE CONSIDERATION FOR PERMANENT FLOWERING, EJPAU 5(1), #02.
Available Online: http://www.ejpau.media.pl/volume5/issue1/horticulture/abs-02.html

THE APPLICATION OF RAPD MARKERS FOR GENETIC STUDIES ON FRAGARIA X ANANASSA DUCH. (STRAWBERRY) WITH DUE CONSIDERATION FOR PERMANENT FLOWERING

Elżbieta Kaczmarska, Jerzy A. Hortyński

 

ABSTRACT



The level of genetic diversity between 16 cultivars of strawberry (Fragaria x ananassa Duch.) and the wild species of Fragaria virginiana Duch. was studied on the basis of the analysis of their DNA (RAPD reaction – randomly amplified polymorphic DNA). Six 10-nucleotide primers generated jointly 354 bands, of which 94.8% were polymorphic and 5.2% monomorphic. The analysis of interrelations was carried out on the basis of Dice similarity matrix. Presentation of relationship between the analysed genotypes on the dendrogram obtained by means of the unweighted pair-group method with arithmetic average (UPGMA).

Key words: bulked segregant analysis (BSA), photoperiodism, Fragaria x ananassa, genetic similarity, RAPD markers.