Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

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Nowak B. , Miczyński K. 2002. THE COURSE AND EFFICIENCY OF ORGANOGENESIS ON LEAF EXPLANTS OF PLUM ´WĘGIERKA ZWYKŁA´ (PRUNUS DOMESTICA L.) INDUCED BY CYTOKININS., EJPAU 5(1), #02.
Available Online: http://www.ejpau.media.pl/volume5/issue1/biotechnology/abs-02.html

ABSTRACT

The effects of organogenesis on in vitro leaf explants of plum ‘Węgierka Zwykła’ cultured on media with either TDZ or BAP were compared. TDZ was applied at concentration of 0.5 – 1.65 mg/l in combination with 2,4 – D, and BAP at concentration of 3.0 or 5.0 mg/l with one of the auxins: IBA, NAA or 2,4 – D. As the result two different ways of organogenesis were observed: the efficient (60%-72%) indirect organogenesis induced by TDZ, and direct organogenesis, or with only very small callus formation on media supplied with BAP. Explants cultured on media with TDZ produced big masses of callus followed by differentiation of numerous adventitious buds in clusters while on media containing BAP only single buds of smaller size were formed.

Key words: BAP, cytokinin, organogenesis, plum, Prunus domestica L., TDZ.

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