Available Online: http://www.ejpau.media.pl/volume5/issue1/biotechnology/abs-02.html
THE COURSE AND EFFICIENCY OF ORGANOGENESIS ON LEAF EXPLANTS OF PLUM &ACUTE;WĘGIERKA ZWYKŁA&ACUTE; (PRUNUS DOMESTICA L.) INDUCED BY CYTOKININS.
Barbara Nowak, Kazimierz Miczyński
The effects of organogenesis on in vitro leaf explants of plum ‘Węgierka Zwykła’ cultured on media with either TDZ or BAP were compared. TDZ was applied at concentration of 0.5 – 1.65 mg/l in combination with 2,4 – D, and BAP at concentration of 3.0 or 5.0 mg/l with one of the auxins: IBA, NAA or 2,4 – D. As the result two different ways of organogenesis were observed: the efficient (60%-72%) indirect organogenesis induced by TDZ, and direct organogenesis, or with only very small callus formation on media supplied with BAP. Explants cultured on media with TDZ produced big masses of callus followed by differentiation of numerous adventitious buds in clusters while on media containing BAP only single buds of smaller size were formed.
Key words: BAP, cytokinin, organogenesis, plum, Prunus domestica L., TDZ.