Volume 5
Issue 1
Biotechnology
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Available Online: http://www.ejpau.media.pl/volume5/issue1/biotechnology/abs-01.html
FERMENTATIVE ACTIVITY OF SOMATIC HYBRIDS OF SACCHAROMYCES CEREVISIAE AND CANDIDA SHEHATAE OR PACHYSOLEN TANNOPHILUS
Ewelina Dziuba, Joanna Chmielewska
ABSTRACT
The aim of the research was to obtain interspecific hybrids of distilling yeast and xylose-fermenting yeast and to assess their fermentative activity in media containing xylose. Distilling yeast Saccharomyces cerevisiae D43 and xylose-fermenting yeast Candida shehatae ATCC 58779, Pachysolen tannophilus ATCC 32691 and Pachysolen tannophilus ATCC 60392 were used. Medium containing xylose as the sole carbon source was used for selecting the interspecific hybrids. Incubation temperature (42˚C) and crystal violet (0.01%) were used as additional selection factors for hybrids of the yeast Candida shehatae ATCC 58779 and Pachysolen tannophilus ATCC 32691 or Pachysolen tannophilus ATCC 60392, respectively.
Key words: distilling, distillery yeast, protoplast fusion, xylose fermentation.