Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2002
Volume 5
Issue 1
Topic:
Biotechnology
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Dziuba E. , Chmielewska J. 2002. FERMENTATIVE ACTIVITY OF SOMATIC HYBRIDS OF SACCHAROMYCES CEREVISIAE AND CANDIDA SHEHATAE OR PACHYSOLEN TANNOPHILUS, EJPAU 5(1), #01.
Available Online: http://www.ejpau.media.pl/volume5/issue1/biotechnology/abs-01.html

FERMENTATIVE ACTIVITY OF SOMATIC HYBRIDS OF SACCHAROMYCES CEREVISIAE AND CANDIDA SHEHATAE OR PACHYSOLEN TANNOPHILUS

Ewelina Dziuba, Joanna Chmielewska

 

ABSTRACT



The aim of the research was to obtain interspecific hybrids of distilling yeast and xylose-fermenting yeast and to assess their fermentative activity in media containing xylose. Distilling yeast Saccharomyces cerevisiae D43 and xylose-fermenting yeast Candida shehatae ATCC 58779, Pachysolen tannophilus ATCC 32691 and Pachysolen tannophilus ATCC 60392 were used. Medium containing xylose as the sole carbon source was used for selecting the interspecific hybrids. Incubation temperature (42˚C) and crystal violet (0.01%) were used as additional selection factors for hybrids of the yeast Candida shehatae ATCC 58779 and Pachysolen tannophilus ATCC 32691 or Pachysolen tannophilus ATCC 60392, respectively.

Key words: distilling, distillery yeast, protoplast fusion, xylose fermentation.