Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2002
Volume 5
Issue 1
Topic:
Agronomy
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Tomaszewska–Sowa M. , Drozdowska L. , Szota M. 2002. EFFECT OF CYTOKININS ON IN VITRO MORPHOGENESIS AND PLOIDY OF PEPPER Capsicum annuum L., EJPAU 5(1), #04.
Available Online: http://www.ejpau.media.pl/volume5/issue1/agronomy/abs-04.html

EFFECT OF CYTOKININS ON IN VITRO MORPHOGENESIS AND PLOIDY OF PEPPER CAPSICUM ANNUUM L.

Magdalena Tomaszewska–Sowa, Lucyna Drozdowska, Maria Szota

 

ABSTRACT



The study-compared effect of cytokinins on morphogenesis and ploidy of plants regenerated in vitro from explants of ‘Stanola F1’, ATZ and ATM pepper seeds. The aim of defining the morphogenetic potential of the studied genotypes, half-seed explants consisting the proximal part of the hypocotyl and radicle were put onto the MS medium containing BAP (5.0 mg.dm-3), 2iP (2.5 mg.dm-3), ZEA (2.5 mg.dm-3), TDZ (1.5 mg.dm-3), while MS medium without cytokinins constituted the control. After the initiation period, explants were transferred onto the medium without cytokinins. The effect of the growth regulators to the morphogenetic response of explants was estimated based on the number of explants on which adventitious buds and shoots were developing. The cytokinins applied did not show a significant effect on the development of adventitious buds on pepper explants. However, the ability to regene

Key words: cytokinins, morphogenesis, ploidy, in vitro culture.