Volume 4
Issue 2
Food Science and Technology
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Available Online: http://www.ejpau.media.pl/volume4/issue2/food/abs-15.html
ETHANOL PRODUCTION ON THE MEDIA CONTAINING GLUCOSE AND XYLOSE BY COCULTURE OF PICHIA STIPITIS CCY 39501 AND RESPIRATORY DEFICIENT MUTANT OF SACCHAROMYCES CEREVISIAE V30
Monika Kordowska-Wiater, Zdzisław Targoński
ABSTRACT
Coculture of xylose-fermenting yeast P. stipitis CCY 39501 and respiratory deficient mutant of S. cerevisiae V30 designated as V30 I 40 was used for ethanol fermentation on a medium containing glucose and xylose mixture and compared to P. stipitis monoculture or coculture of P. stipitis and S. cerevisiae V30. Batch fermentations were carried out on a model medium or on a medium containing both sugars derived from direct saccharification of either wheat straw or birch sawdust. The yields obtained were 0.38 g/g, 0.34 g/g and 0.4 g/g for model medium, wheat straw and birch sawdust hydrolysates respectively, after cofermentation of P. stipitis with RD mutant V30 I 40. The results confirmed the application of this coculture for ethanol fermentation of sugars derived from lignocellulosic hydrolysates.
Key words: ethanol fermentation, xylose, lignocellulose, SSF, coculture, respiratory deficient mutant of S. cerevisiae, P. stipitis.