Volume 4
Issue 2
Food Science and Technology
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Available Online: http://www.ejpau.media.pl/volume4/issue2/food/abs-13.html
THE INFLUENCE OF CULTURE CONDITIONS ON ADHESION OF LISTERIA MONOCYTOGENES TO HEXADECANE
Waldemar D±browski, Robert Iwański, Katarzyna Czeszejko, Dagmara Mędrala
ABSTRACT
Results of various culture conditions on adhesion of Listeria monocytogenes strains to hexadecane are presented. Listeria strains cultured in optimal conditions adhered to this hydrophobic carbohydrate to a small extent. It reveals that their cell walls have hydrophilic properties. Application of low-nutrient medium, incubation at low temperature, acidification of medium to pH 5 and addition of 8% of NaCl caused transformation of strains into hydrophobic. Growing in vacuum conditions produced the opposite effect – all strains were extremely hydrophilic. Changes of strain properties from hydrophilic into hydrophobic affected analysed strains to a different degree. One of tested strains continued to be hydrophilic regardless of culture conditions. The other – a standard strain isolated from patient – became hydrophobic most frequently.
Key words: hydrophobicity, Listeria monocytogenes.