Volume 4
Issue 2
Fisheries
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Available Online: http://www.ejpau.media.pl/volume4/issue2/fisheries/abs-03.html
INCUBATION TEMPERATURE EFFECTS ON THE DEVELOPMENT OF HATCHING GLAND CELLS IN IDE, LEUCISCUS IDUS (L.)
Jacek Rechulicz
ABSTRACT
The ide eggs were incubated in the laboratory in three temperature ranges: 11–13, 15–16, and 18–20°C. Throughout the entire period of incubation, the live eggs were observed and samples were collected for histological assays. Microscopic slides were made to record the timing of appearance of hatching gland cells. The distribution, number, and size of the hatching gland cells, relative to incubation temperature, were determined. The cells appeared at the earliest (after 75 h of incubation) in the eggs incubated at 18–20°C and took the longest to appear (145 h) in those eggs incubated at the lowest temperature. The hatching gland cells were observed to be present only in the anterior part of the ide embryo and were lacking in the caudal part. Depending on the temperature, an embryo showed the presence of 200 to 390 hatching gland cells. The largest cells, having 29.30 µm mean length, 24.54 µm mean height, and 9694.58 µm3 mean volume, were typical of those embryos
Key words: ide, egg incubation, hatching gland cells (HGCs).