Volume 4
Issue 2
Animal Husbandry
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Available Online: http://www.ejpau.media.pl/volume4/issue2/animal/abs-06.html
POLYMORPHISM VS. EXPRESSION OF PROTEIN CODING GENES IN JAPANESE QUAIL
Antoni Brodacki, Alicja Wójcik
ABSTRACT
The study covered 97 Japanese quails of various genotypes of the protein referred to as preactin (PAK). The birds of AA genotype had a single, intensively stained, fast–migrating band; the BB genotypes had a single, slow–migrating, also intensively stained band, whereas the AB birds had two bands. The protein concentration, established basing on the area and height of peaks, was significantly higher in the BB homozygotes than in the AA ones, which appeared also in the form of more intensive staining of the slow–migrating band than that of the fast–migrating band of the AB heterozygotes. The differences, however, were insignificant. The protein level of heterozygotes in the sub–region, calculated as the sum of two bands, was more than 50% higher than in either of the homozygous forms. The differences, confirmed statistically, can demonstrate that the genotype determining the proteins influences the expression of the genes coding particular proteins.
Key words: polymorphism, proteins, gene expression.