Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

Copyright © Wydawnictwo Akademii Rolniczej we Wroclawiu, ISSN 1505-0297

Fordoński G. , Łapiński M. , Pszczółkowska A. , Kulik T. 2001. IDENTIFYING Fusarium avenaceum AND Fusarium culmorum IN SELECTED LEGUMES AND CEREALS WITH THE PCR METHOD, EJPAU 4(2), #02.
Available Online: http://www.ejpau.media.pl/volume4/issue2/agronomy/abs-02.html

ABSTRACT

The paper showed the applicability of the PCR method to an early identification of Fusarium avenaceum and Fusarium culmorum in infected tissues of selected crops. The polymerisation chain reaction used species-specific SCAR primers. There was observed a discrepancy between the size of multiplied DNA of Fusarium avenaceum fragment and that of Fa-U17f 5’, Fa-U17r earlier described by authors of primers. The present research product size for F. avenaceum was 950 bp, while for F. culmorum - 472 bp which confirmed the reports by other authors.

Key words: Fusarium avenaceum, Fusarium culmorum, DNA, PCR, SCAR primers.

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