Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2000
Volume 3
Issue 1
Topic:
Agronomy
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Lahuta L. , Górecki R. , Michalczyk D. , Piotrowicz-Cieślak A. 2000. Alpha - D-GALACTOSIDASE ACTIVITY IN STORED YELLOW LUPIN (Lupinus luteus L.) SEEDS, EJPAU 3(1), #05.
Available Online: http://www.ejpau.media.pl/volume3/issue1/agronomy/abs-05.html

ALPHA - D-GALACTOSIDASE ACTIVITY IN STORED YELLOW LUPIN (LUPINUS LUTEUS L.) SEEDS

Lesław B. Lahuta, Ryszard J. Górecki, Dariusz Michalczyk, Agnieszka I. Piotrowicz-Cieślak

 

ABSTRACT



The activity of alpha-D-galactosidase and contents of soluble saccharides were studied in ‘Juno’ yellow lupin (Lupinus luteus L.) seeds stored. Seeds harvested at three stages of maturity (15, 25, 35 days after flowering - DAF) were stored at –21°C for two years (frozen immediately after harvest) or were dried after harvest to 8% of water content and stored at +20°C for 5 and 6 years. A high alpha-D-galactosidase activity in maturing and mature seeds could have been one of the causes of decreasing viability and vigour of the seeds stored. The hydrolysis of raffinose oligosaccharides decreased the ratio of these saccharides to sucrose. The decrease in the content of raffinose oligosaccharides was accompanied by an increase in galactosyl cyclitol contents.

Key words: alpha-D-galactosidase, yellow lupin, seed storage, raffinose, cyclitols.