Available Online: http://www.ejpau.media.pl/volume3/issue1/agronomy/abs-05.html
ALPHA - D-GALACTOSIDASE ACTIVITY IN STORED YELLOW LUPIN (LUPINUS LUTEUS L.) SEEDS
Lesław B. Lahuta, Ryszard J. Górecki, Dariusz Michalczyk, Agnieszka I. Piotrowicz-Cieślak
The activity of alpha-D-galactosidase and contents of soluble saccharides were studied in Juno yellow lupin (Lupinus luteus L.) seeds stored. Seeds harvested at three stages of maturity (15, 25, 35 days after flowering - DAF) were stored at 21°C for two years (frozen immediately after harvest) or were dried after harvest to 8% of water content and stored at +20°C for 5 and 6 years. A high alpha-D-galactosidase activity in maturing and mature seeds could have been one of the causes of decreasing viability and vigour of the seeds stored. The hydrolysis of raffinose oligosaccharides decreased the ratio of these saccharides to sucrose. The decrease in the content of raffinose oligosaccharides was accompanied by an increase in galactosyl cyclitol contents.
Key words: alpha-D-galactosidase, yellow lupin, seed storage, raffinose, cyclitols.