Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

Copyright © Wydawnictwo Akademii Rolniczej we Wroclawiu, ISSN 1505-0297

Sliwińska E. , Pedersen H. 1999. FLOW CYTOMETRIC ANALYSIS OF NUCLEAR REPLICATION STAGES DURING GERMINATION OF SUGAR-BEET SEEDS DIFFERENT IN VIGOUR, EJPAU 2(1), #01.
Available Online: http://www.ejpau.media.pl/volume2/issue1/agronomy/abs-01.html

ABSTRACT

The nuclear replication stages in dry and germinating seeds of two sugar-beet cultivars were determined by flow cytometry. For each cultivar two seed lots of similar, high germination capacity, but different in seedling length vigour index (SLVI; applied as quality marker) were studied. Analyses were performed every 12 hours during 72 hours of germination and early seedling growth at 20^oC and 15^oC. Before start of germination, in all seed lots, about 90% of cells were arrested in the G₁ phase of the cell cycle. The proportion of G₁ cells decreased with time of germination, faster in higher quality seed lots than in those of lower quality. The G₂/G₁ ratio in dry seeds was slightly higher for the seeds of higher SLVI, and it increased during germination in all seed lots. However, the difference between higher and lower quality seeds of the same cultivar did not become more distinct during germination. The differentiation in cell cycle activity wa

Key words: Beta vulgaris L., cell cycle, DNA replication, flow cytometry, germination, seed, vigour.

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