Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural
sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS
Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing
UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2003
Volume 6
Issue 1
Volume 6
Issue 1
Topic:
Food Science and Technology
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Copyright © Wydawnictwo Uniwersytetu Przyrodniczego we Wrocławiu, ISSN 1505-0297
Leśnierowski G. , Kijowski J. , Stangierski J. 2003. DSC, SDS-PAGE AND SPECTROPHOTOMETRY FOR CHARACTERIZATION OF MODIFIED LYSOZYME, EJPAU 6(1), #11.
Available Online: http://www.ejpau.media.pl/volume6/issue1/food/abs-11.html
Available Online: http://www.ejpau.media.pl/volume6/issue1/food/abs-11.html
DSC, SDS-PAGE AND SPECTROPHOTOMETRY FOR CHARACTERIZATION OF MODIFIED LYSOZYME
Grzegorz Leśnierowski, Jacek Kijowski, Jerzy Stangierski
ABSTRACT
The aim of the study was an attempt to apply selected analytical methods for the evaluation of preparations of modified lysozyme. Lysozyme isolated from hen egg white was modified using thermal, thermal-chemical, chemical and membrane methods and subsequently the obtained preparations were evaluated with the use of the spectrophotometric method, as well as electrophoresis and differential scanning calorimetry (DSC).
Key words:
differential scanning calorimetry (DSC), spectrophotometry, electrophoresis, lysozyme modification, lysozyme dimer, hydrolytic activity.