Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2004
Volume 7
Issue 2
Topic:
Biotechnology
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Kawa-Rygielska J. 2004. OBTAINING HYBRIDS OF DISTILLERY YEASTS CHARACTERISED BY THE ABILITY OF FERMENTING STARCH, EJPAU 7(2), #02.
Available Online: http://www.ejpau.media.pl/volume7/issue2/biotechnology/abs-02.html

OBTAINING HYBRIDS OF DISTILLERY YEASTS CHARACTERISED BY THE ABILITY OF FERMENTING STARCH

Joanna Kawa-Rygielska

 

ABSTRACT



The aim of the work was to obtain fusants of Saccharomyces and Schwanniomyces yeast strains. Biological material was composed of Schwanniomyces occidentalis ATCC 48086, Saccharomyces cerevisiae "malt", Saccharomyces diastaticus ATCC 13007. The use of natural markers, such as the growth of Schwanniomyces strain in medium with cyclohexymide and the growth of Saccharomyces yeast at 400C helped select 26 fusion products. Genetic analysis of DNA using the PCR method showed the presence of three hybrids containing DNA of both parental strains.

Key words: yeast, protoplast fusion, DNA..