Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2004
Volume 7
Issue 2
Topic:
Biology
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Białkowska J. , Dietrich G. , Demianowicz W. , Glogowski J. 2004. EVALUATION OF CASA SYSTEM FOR PREDICTING THE MOTILITY OF ZEBRA MUSSEL (DREISSENA POLYMORPHA PALLAS) SPERMATOZOA, EJPAU 7(2), #4.
Available Online: http://www.ejpau.media.pl/volume7/issue2/biology/abs-4.html

EVALUATION OF CASA SYSTEM FOR PREDICTING THE MOTILITY OF ZEBRA MUSSEL (DREISSENA POLYMORPHA PALLAS) SPERMATOZOA

Joanna Białkowska, Grzegorz J. Dietrich, Wiesław Demianowicz, Jan Glogowski

 

ABSTRACT



A computer – aided semen analyzer (CASA) was used for monitoring some motility parameters of zebra mussel sperm after spawning for up to 1,5h at 22°C and after 24 hours of storage at 8°C. The mean percentage of motility (MOT) for sperm shortly after spawning was 70±2.0 %, VSL = 31.8±3.0 µm s-1, and VCL= 110.2±6.4 µm s-1. The motility expressed as LIN was 27.7±2.0 %, STR=44.5±4.8 %, ALH=8.7±2.0 µm, BCF=6.8±2.0 Hz. The data demonstrate a consistent decrease in VSL (12.8± 4.0), VCL (57.4± 5.7), LIN (15.8± 3.0) and ALH (1.1± 0.23) values after 24 hours. No significant differences were found for % of MOT, BCF and STR parameters values during the time of experiment. We found CASA analyzer as a proper tool helpful to discerning the quality of zebra mussel semen samples.

Key words: Dreissena polymorpha, sperm motility, CASA..