Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

Copyright © Wydawnictwo Uniwersytetu Przyrodniczego we Wrocławiu, ISSN 1505-0297

Musiał I. , Rymowicz W. , Cibis E. 2004. OPTIMIZATION OF SINGLE-CELL-BIOMASS PRODUCTION BY YARROWIA LIPOLYTICA USING RESPONSE SURFACE METHODOLOGY AND PULSE METHOD, EJPAU 7(1), #02.
Available Online: http://www.ejpau.media.pl/volume7/issue1/biotechnology/abs-02.html

ABSTRACT

The medium for fodder yeast production from crude rapeseed oil by the strain Yarrowia lipolytica A-101 was optimized using both the response surface methodology and the pulse method. The 2⁴ central composite experimental design was used to study the combined effect of the medium components, such as crude rapeseed oil, ammonium sulphate, magnesium sulphate and yeast extract, on the biomass concentration and yield. To maximize the concentration of the biomass the optimal values of the medium components were determined with the two methods for the independent variables. Both methods yielded similar results. Thus the optimized concentrations of the medium components amounted to 25.7; 4.2; 0.12; 0.28 and 2.1 gL^-1 for crude rapeseed oil, ammonium sulphate, magnesium sulphate, potassium dihydrogen phosphate and yeast extract, respectively.

Key words: medium optimization, rapeseed oil, Yarrowia lipolytica.

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