Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

Copyright © Wydawnictwo Uniwersytetu Przyrodniczego we Wrocławiu, ISSN 1505-0297

Sip A. , Grajek W. 2003. BIOSYNTHESIS OF DIVERCIN BY CARNOBACTERIUM DIVERGENS AS7 IN CONTINUOUS HIGH CELL DENSITY CULTURES, EJPAU 6(1), #02.
Available Online: http://www.ejpau.media.pl/volume6/issue1/biotechnology/abs-02.html

ABSTRACT

Continuous cultures of Carnobacterium divergens AS7 with cell recycling were characterized with respect to their growth kinetics, fermentative activity and ability to biosynthesize the anti-listerial bacteriocin divercin. The experimental variables were dilution rates from 0.14 h^-1 to 0.36 h^-1 and a two-level recycling rate. The experiments demonstrated that dilution rate and cell recycling strongly influenced cell density in a bioreactor. With an increase in dilution rate, an increase in cell density was also observed. At D=0.36 h^-1 the maximum cell density of 48 g d.m. l^-1 was obtained. With long fermentation time and high cell density, the number of dead cells rapidly increased. In these experiments, when cell density increased 10-fold, the number of viable cells increased only 2-fold. It was found that divercin biosynthesis was reciprocally correlated to dilution rate. The greater dilution rate, the lower the volumetric productivity of

Key words: Divercin, Carnobacterium divergens, membrane bioreactor, high cell density culture.

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