Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2001
Volume 4
Issue 2
Topic:
Food Science and Technology
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Sip A. , Grajek W. 2001. TRANSFER OF DIVERCIN THROUGH ULTRAFILTRATION MEMBRANES AFTER TREATMENT WITH DETERGENTS AND ORGANIC SOLVENTS, EJPAU 4(2), #08.
Available Online: http://www.ejpau.media.pl/volume4/issue2/food/abs-08.html

TRANSFER OF DIVERCIN THROUGH ULTRAFILTRATION MEMBRANES AFTER TREATMENT WITH DETERGENTS AND ORGANIC SOLVENTS

Anna Sip, Włodzimierz Grajek

 

ABSTRACT



The ultrafiltration of post-culture fluids proved that divercin occurred in the form of large macroaggregates with the molecular weight over 100 kDa. Addition of detergents: Tween 80, Nonidet P-40 and SDS, and alcohols: ethanol and isopropanol into supernatants from post-culture fluids caused partial disaggregation of divercin. The better effect was obtained when Tween 80 was introduced directly into culture medium. However, other detergents and both alcohols showed a toxic effect to the bacteria and reduced the divercin production in C. divergens culture. The ultrafiltration yield of divercin depended on the type of membranes and treatment of divercin-containing extracts with detergents and alcohols. The cellulose acetate membranes were more permeable for divercin that made from polyethersulfone. The highest divercin disaggregation in active extracts, made in evidence by the improvement of divercin transfer through membrane cascade, was obtained as result of 1 % of SDS and Nonidet

Key words: divercin, bacteriocin, ultrafiltration, detergents, alcohols, Carnobacterium divergens, aggregation, polyethersulfone membrane, cellulose acetate membrane.